A point-of-care (POC) device to enable de-centralized diagnostics can effectively reduce the time to treatment, especially in case of infectious diseases. However, many of the POC solutions presented so far do not comply with the ASSURED (affordable, sensitive, specific, user-friendly, rapid and robust, equipment free, and deliverable to users) guidelines that are needed to ensure their on-field deployment. Herein, we present the proof of concept of a self-powered platform that operates using the analysed fluid, mimicking a blood sample, for early stage detection of HIV-1 infection. The platform contains a smart interfacing circuit to operate an ultra-sensitive electrolyte-gated field-effect transistor (EGOFET) as a sensor and facilitates an easy and affordable readout mechanism. The sensor transduces the bio-recognition event taking place at the gate electrode functionalized with the antibody against the HIV-1 p24 capsid protein, while it is powered via paper-based biofuel cell (BFC) that extracts the energy from the analysed sample itself. The self-powered platform is demonstrated to achieve detection of HIV-1 p24 antigens in fM range, suitable for early diagnosis. From these developments, a cost-effective digital POC device able to detect the transition from “healthy” to “infected” state at single-molecule precision, with no dependency on external power sources while using minimal components and simpler approach, is foreseen.

启用分散诊断的即时医疗（POC）设备可以有效地缩短治疗时间，尤其是在传染性疾病的情况下。但是，到目前为止，提出的许多POC解决方案都不符合确保其现场部署所需的ASSURED（价格适中，敏感，特定，用户友好，快速且健壮，无需设备且可交付给用户）准则。 。在此，我们介绍了一种自供电平台的概念证明，该平台可使用分析液模拟血液样本来进行HIV-1感染的早期检测。该平台包含一个智能接口电路，可将超灵敏的电解质门控场效应晶体管（EGOFET）用作传感器，并促进一种简单且价格合理的读出机制。传感器转导在用抗HIV-1 p24衣壳蛋白抗体功能化的栅电极上发生的生物识别事件，而传感器通过纸基生物燃料电池（BFC）供电，该生物燃料电池从被分析的样品本身中提取能量。经证明，该自动平台可实现在fM范围内检测HIV-1 p24抗原，适合早期诊断。通过这些发展，可以预见到一种经济有效的数字POC设备，该设备能够以单分子精度检测从“健康”状态到“感染”状态的转变，而无需依赖外部电源，同时使用最少的组件和更简单的方法。经证明，该自动平台可实现在fM范围内检测HIV-1 p24抗原，适合早期诊断。通过这些发展，可以预见到一种经济有效的数字POC设备，该设备能够以单分子精度检测从“健康”状态到“感染”状态的转变，而无需依赖外部电源，同时使用最少的组件和更简单的方法。经证明，该自动平台可实现在fM范围内检测HIV-1 p24抗原，适合早期诊断。通过这些发展，可以预见到一种经济有效的数字POC设备，该设备能够以单分子精度检测从“健康”状态到“感染”状态的转变，而无需依赖外部电源，同时使用最少的组件和更简单的方法。