Members of Bacillaceae family are of major interest in medical industry due to vast antimicrobial peptides they produce as therapeutic agents. For decades, synthetic and natural occurring antibiotics have been used to treat infectious diseases, but heavy dependence on these drugs has led to significant drawbacks which propel continuous development of new antibiotics generation. Recent findings have shown several bacteriocins of Bacillaceae as promising alternatives to the conventional drugs to combat the emergence of new drug-resistant pathogens. In this present review, Bacillaceae bacteriocins’ classification such as lantibiotics and thiazole/oxazole-modified microcins as well as their biochemical characterization such as sensitivity to enzymes, temperature, pH and chemicals are described. This article enlightens on the medical application of several Bacillaceae bacteriocins emphasizing those that underwent and on-going preclinical trials. This review also discusses the development of Bacillaceae bacteriocins production, focusing strains selection and fermentation factors such as inocula size, medium (carbon, nitrogen, minerals sources), temperature, pH, agitation and aeration rate, dissolved oxygen tension (DOT), fermentation time, inducers and mode of operation via various statistical methods for their optimization. It also highlights recent advance in the production of bioengineered and recombinant bacteriocins in bioreactors system which are rarely disclosed in literature.

中文翻译：

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杆菌科细菌素生产的生物制造工艺

芽孢杆菌科的成员由于其作为治疗剂而产生的大量抗菌肽而在医学界引起了广泛关注。几十年来，合成和天然存在的抗生素已被用于治疗传染病，但是对这些药物的严重依赖已导致明显的弊端，从而推动了新一代抗生素的持续开发。最近的发现表明，芽孢杆菌属的几种细菌素可作为常规药物的有希望的替代品，以对抗新出现的耐药性病原体的出现。在本综述中，描述了芽孢杆菌属细菌素的分类，如羊毛硫抗生素和噻唑/恶唑修饰的微素，以及它们的生化特征，如对酶，温度，pH和化学物质的敏感性。本文着重介绍了几种芽孢杆菌属细菌素的医学应用，重点介绍了那些正在进行和正在进行的临床前试验的细菌素。这篇综述还讨论了芽孢杆菌属细菌素生产的发展，重点关注菌株的选择和发酵因素，例如接种量，培养基（碳，氮，矿物质来源），温度，pH，搅拌和曝气速率，溶解氧张力（DOT），发酵时间，诱导程序和操作模式通过各种统计方法进行优化。它还强调了在生物反应器系统中生产生物工程和重组细菌素的最新进展，这在文献中很少披露。这篇综述还讨论了芽孢杆菌属细菌素生产的发展，重点关注菌株的选择和发酵因素，例如接种量，培养基（碳，氮，矿物质来源），温度，pH，搅拌和曝气速率，溶解氧张力（DOT），发酵时间，诱导程序和操作模式通过各种统计方法进行优化。它还强调了在生物反应器系统中生产生物工程和重组细菌素的最新进展，这在文献中很少披露。这篇综述还讨论了芽孢杆菌属细菌素生产的发展，重点关注菌株的选择和发酵因素，例如接种量，培养基（碳，氮，矿物质来源），温度，pH，搅拌和曝气速率，溶解氧张力（DOT），发酵时间，诱导程序和操作模式通过各种统计方法进行优化。它还强调了在生物反应器系统中生产生物工程和重组细菌素的最新进展，这在文献中很少披露。通过各种统计方法优化诱导剂和操作方式。它还强调了在生物反应器系统中生产生物工程和重组细菌素的最新进展，这在文献中很少披露。通过各种统计方法优化诱导剂和操作方式。它还强调了在生物反应器系统中生产生物工程和重组细菌素的最新进展，这在文献中很少披露。