Abstract

The composite hydrogel was prepared with cellulose and flaxseed gum and characterized by fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Brunauer–Emmitt–Teller (BET) and thermogravimetric analysis (TGA). The swelling property, drug adsorption, biocompatibility and hemostatic function of composite hydrogel were investigated. The composite hydrogel had the pore structure and exhibited high stability with a thermal decomposition temperature of 332.66 °C. And the composite hydrogel showed excellent swelling capability with a moisture expansivity of over 200%, and with a drug adsorption capacity of 7.27 ± 0.15 mg/g. Mechanical properties tests showed that as the proportion of flaxseed gum increased, the ability to withstand pressure of hydrogel was improved. In addition, MTT assay, flow cytometry analysis, and in vivo toxicological evaluation suggested that composite hydrogel had good biocompatibility. Moreover, hemostatic potential assay and wound healing were made in order to evaluate the effect of hemostasis and wound healing of composite hydrogel and our results indicated that the composite hydrogel with cellulose and flaxseed gum could be effective to promote hemostatic and wound healing function. All in all, the great properties exhibited by the composite hydrogel could make it a potential candidate as biomaterial in bleeding and wound treatment.

Graphic abstract

摘要

用纤维素和亚麻籽胶制备复合水凝胶，并通过傅里叶变换红外光谱（FTIR），X射线衍射（XRD），Brunauer-Emmitt-Teller（BET）和热重分析（TGA）进行表征。研究了复合水凝胶的溶胀性能，药物吸附，生物相容性和止血功能。该复合水凝胶具有孔结构并显示出高稳定性，其热分解温度为332.66℃。并且该复合水凝胶显示出优异的溶胀能力，水分膨胀率超过200％，药物吸附能力为7.27±0.15 mg / g。力学性能测试表明，随着亚麻籽胶比例的增加，水凝胶承受压力的能力得到提高。此外，MTT分析，流式细胞仪分析，体内毒理学评价表明复合水凝胶具有良好的生物相容性。此外，还进行了止血潜力测定和伤口愈合，以评估复合水凝胶的止血和伤口愈合的效果，我们的结果表明，含有纤维素和亚麻籽胶的复合水凝胶可以有效地促进止血和伤口愈合功能。总而言之，复合水凝胶展现出的出色性能使其成为出血和伤口治疗中生物材料的潜在候选者。为了评估复合水凝胶的止血和伤口愈合的效果，进行了止血电位测定和伤口愈合，我们的结果表明，含有纤维素和亚麻籽胶的复合水凝胶可以有效地促进止血和伤口愈合功能。总而言之，复合水凝胶展现出的出色性能使其成为出血和伤口治疗中生物材料的潜在候选者。为了评估复合水凝胶的止血和伤口愈合的效果，进行了止血电位测定和伤口愈合，我们的结果表明，含有纤维素和亚麻籽胶的复合水凝胶可以有效地促进止血和伤口愈合功能。总而言之，复合水凝胶展现出的出色性能使其成为出血和伤口治疗中生物材料的潜在候选者。

图形摘要