A traceless clean-up method coupled with gas chromatography and mass spectrometry for analyzing polycyclic aromatic hydrocarbons in complex plant leaf matrices.,Analyst

当前位置： X-MOL 学术 › Analyst › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

A traceless clean-up method coupled with gas chromatography and mass spectrometry for analyzing polycyclic aromatic hydrocarbons in complex plant leaf matrices.
Analyst ( IF 3.6 ) Pub Date : 2020-04-01 , DOI: 10.1039/d0an00128g
Xiangzi Jin ₁ , Han Yeong Kaw , Huijie Li , Zhao Wang , Jinhua Zhao , Xiangfan Piao , Donghao Li , Dongri Jin , Miao He

Affiliation

This study developed a traceless clean-up method by combining solid phase extraction (SPE) with gas purge-microsyringe extraction (GP-MSE) to purify sample extracts for the determination of polycyclic aromatic hydrocarbons (PAHs) in plant leaves. SPE exhibited good purification performance for the removal of polar lipids, while the GP-MSE technique effectively eliminated less-volatile lipids hence realizing zero damage to the instrument, and significantly improved the peak tailings. After ultrasonic extraction, the combined two-step clean-up procedure successfully removed over 99% of lipids from nineteen types of tree leaves, and PAHs in tree leaves were determined by GC-MS. The relative standard deviations (RSDs) for intra-day (n = 3) and inter-day (n = 3) analyses of PAHs in spiked willow samples were in the range of 0.8%-12.1% and 4.7%-15.3%, respectively. The recoveries of PAHs from spiked willow extracts ranged from 74 to 90%, with an average of 86%. The method detection limit (MDL) of PAHs in tree leaves ranged from 0.1 to 4.9 ng g-1 dry weight. In conclusion, the clean-up method in this study realized the analysis of PAHs in plant leaves with high accuracy, sensitivity and reproducibility. Most importantly, the two-step purification method significantly minimizes damage to the GC-MS system particularly to the column and ion source, which is beneficial to ensure continuous analysis of a large number of samples with good performance.

中文翻译：

一种无痕净化方法，结合气相色谱和质谱分析复杂植物叶片基质中的多环芳烃。

这项研究通过结合固相萃取（SPE）和气体吹扫微注射器萃取（GP-MSE）来纯化样品提取物以测定植物叶片中的多环芳烃（PAHs），从而开发了一种无痕的净化方法。SPE对极性脂质的去除表现出良好的纯化性能，而GP-MSE技术有效消除了挥发性较低的脂质，从而实现了对仪器的零损害，并显着改善了峰拖尾。超声提取后，结合两步净化程序成功地从19种类型的树叶中去除了99％以上的脂质，并通过GC-MS测定了树叶中的PAH。日间（n = 3）和日间（n = 3）分析加标柳树样品中PAH的相对标准偏差（RSD）在0.8％-12.1％和4.7％-15.3％之间，分别。穗状柳提取物中多环芳烃的回收率从74％到90％不等，平均为86％。树叶中PAHs的方法检测限（MDL）为0.1至4.9 ng g-1干重。总之，本研究中的净化方法实现了植物叶片中多环芳烃的分析，具有较高的准确度，灵敏度和重现性。最重要的是，两步纯化方法极大地减少了对GC-MS系统的损害，特别是对色谱柱和离子源的损害，这对于确保连续分析大量具有良好性能的样品是有好处的。本研究中的净化方法实现了植物叶片中PAHs的分析，具有较高的准确度，灵敏度和重现性。最重要的是，两步纯化方法极大地减少了对GC-MS系统的损害，特别是对色谱柱和离子源的损害，这对于确保连续分析大量具有良好性能的样品是有好处的。本研究中的净化方法实现了植物叶片中PAHs的分析，具有较高的准确度，灵敏度和重现性。最重要的是，两步纯化方法极大地减少了对GC-MS系统的损害，特别是对色谱柱和离子源的损害，这对于确保连续分析大量具有良好性能的样品是有好处的。

更新日期：2020-02-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11