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Phytohormone profiles are strongly altered during induction and symptom development of the physiological ripening disorder berry shrivel in grapevine.
Plant Molecular Biology ( IF 5.1 ) Pub Date : 2020-02-18 , DOI: 10.1007/s11103-020-00980-6
Michaela Griesser 1 , Stefania Savoi 2 , Suriyan Supapvanich 3 , Petre Dobrev 4 , Radomira Vankova 4 , Astrid Forneck 1
Affiliation  

The process of grape berry ripening follows three phases with distinct metabolic processes and complex regulations via phytohormones. The physiological ripening disorder berry shrivel (BS) is characterized by reduced sugar accumulation, low anthocyanin contents, and high acidity in affected berries. The processes leading to BS induction are unknown, but recent transcriptional data on reduced expression of switch genes hint towards a disturbed ripening onset. Herein we investigated the phytohormone composition throughout grape berry ripening in healthy and BS berries in Vitis vinifera L. cultivar Blauer Zweigelt. Thereby we hypothesize that phytohormones are key players for BS induction and suppress the expression of switch genes at veraison. The presented metabolomics and RNAseq data describe two distinct phytohormone profiles in BS berries, differing between pre- and post-veraison with a clear ethylene precursor (aminocyclopropane-1-carboxylic acid, ACC) peak before veraison. Exogenous application of ACC led to BS symptoms, while ethephone application led to berry abscission. During post-veraison, we observed high ABA-glucose ester (ABA-GE) and low indole-3-acetate aspartate (IAA-Asp) and isopentenyladenine (iP) contents in BS berries and the transcriptional induction of several phytohormone pathways. The presented descriptive data provide valuable knowledge to further decipher the role of phytohormones in BS induction and BS symptom development.

中文翻译:

在生理成熟障碍葡萄枯萎病的诱导和症状发展过程中,植物激素的分布发生了很大的变化。

葡萄浆果的成熟过程分为三个阶段,这些阶段具有独特的代谢过程和通过植物激素的复杂调控。生理成熟障碍浆果枯萎病(BS)的特征是受累浆果中的糖积累减少,花青素含量低和酸度高。导致BS诱导的过程是未知的,但有关开关基因表达降低的最新转录数据暗示了成熟成熟的开始。在这里,我们调查了在葡萄和健康浆果中的葡萄果实成熟过程中植物激素的组成。因此,我们假设植物激素是BS诱导的关键因素,并确实抑制了开关基因的表达。呈现的代谢组学和RNAseq数据描述了BS浆果中两种不同的植物激素谱,检验前后,在检验之前有一个清晰的乙烯前体(氨基环丙烷-1-羧酸,ACC)峰,因此有差异。外源使用ACC会导致BS症状,而使用Ephone则会导致浆果脱落。在验证后,我们观察到了BS浆果中的高ABA-葡萄糖酸酯(ABA-GE)和低吲哚-3-乙酸天冬氨酸(IAA-Asp)和异戊烯腺嘌呤(iP)含量,以及几种植物激素途径的转录诱导。所提供的描述性数据提供了宝贵的知识,可进一步了解植物激素在BS诱导和BS症状发展中的作用。在验证后,我们观察到了BS浆果中的高ABA-葡萄糖酸酯(ABA-GE)和低吲哚-3-乙酸天冬氨酸(IAA-Asp)和异戊烯腺嘌呤(iP)含量,以及几种植物激素途径的转录诱导。所提供的描述性数据提供了宝贵的知识,可进一步了解植物激素在BS诱导和BS症状发展中的作用。在验证后,我们观察到了BS浆果中的高ABA-葡萄糖酸酯(ABA-GE)和低吲哚-3-乙酸天冬氨酸(IAA-Asp)和异戊烯腺嘌呤(iP)含量,以及几种植物激素途径的转录诱导。所提供的描述性数据提供了宝贵的知识,可进一步了解植物激素在BS诱导和BS症状发展中的作用。
更新日期:2020-04-22
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