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Angiotensin‐I‐converting enzyme (ACE)‐inhibitory peptides from Thai jasmine rice bran protein hydrolysates
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-02-18 , DOI: 10.1111/ijfs.14495 Orapa Suwannapan 1 , Kwanruedee Wachirattanapongmetee 2 , Supawan Thawornchinsombut 2, 3 , Somporn Katekaew 1
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-02-18 , DOI: 10.1111/ijfs.14495 Orapa Suwannapan 1 , Kwanruedee Wachirattanapongmetee 2 , Supawan Thawornchinsombut 2, 3 , Somporn Katekaew 1
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Rice bran protein hydrolysate (<50 kDa RBPH) from Thai jasmine variety demonstrating a high Angiotensin I converting enzyme (ACE) inhibitory activity was purified and characterised. ACE inhibitory peptides were obtained from a two‐step purification process: gel filtration and preparative reverse‐phase high‐performance chromatography (RP‐HPLC) and then identified by mass spectrometer hybrid quadrupole‐time‐of‐flight. A novel peptide GSGYF in the RBPH was firstly identified and found to have a partial sequence homology of Oryza sativa Japonica Group. This sequence was further synthesised to exhibit as good an inhibition potency with IC50 value of 2.11 µg mL−1 as Captopril (1.15 µg mL−1). The cytotoxicity test revealed that this RBPH is non‐toxic against Vero cells. In addition, the <50 kDa RBPH was resistant to in vitro digestion by pepsin and trypsin. These findings suggest that the RBPH containing ACE inhibitory peptides is likely to be safer and healthier than synthetic drugs and can be an effective food supplement for lowering blood pressure.
中文翻译：
来自泰国茉莉米糠蛋白水解物的血管紧张素转换酶（ACE）抑制肽
纯化和表征了泰国茉莉花品种的米糠蛋白水解产物（<50 kDa RBPH），它具有较高的血管紧张素转换酶（ACE）抑制活性。ACE抑制肽可通过两步纯化过程获得：凝胶过滤和制备型反相高效色谱（RP-HPLC），然后通过质谱仪混合四极杆飞行时间进行鉴定。首次鉴定了RBPH中的一种新型肽GSGYF，发现其与水稻组具有部分序列同源性。该序列进一步合成，以显示出良好的抑制效力与IC 50值2.11微克毫升-1如卡托普利（1.15微克毫升-1）。细胞毒性测试表明该RBPH对Vero细胞无毒。此外，<50 kDa的RBPH对胃蛋白酶和胰蛋白酶的体外消化具有抗性。这些发现表明，含RBPH的ACE抑制肽可能比合成药物更安全，更健康，并且可以作为降低血压的有效食品补充剂。
更新日期：2020-02-18
中文翻译：
来自泰国茉莉米糠蛋白水解物的血管紧张素转换酶（ACE）抑制肽
纯化和表征了泰国茉莉花品种的米糠蛋白水解产物（<50 kDa RBPH），它具有较高的血管紧张素转换酶（ACE）抑制活性。ACE抑制肽可通过两步纯化过程获得：凝胶过滤和制备型反相高效色谱（RP-HPLC），然后通过质谱仪混合四极杆飞行时间进行鉴定。首次鉴定了RBPH中的一种新型肽GSGYF，发现其与水稻组具有部分序列同源性。该序列进一步合成，以显示出良好的抑制效力与IC 50值2.11微克毫升-1如卡托普利（1.15微克毫升-1）。细胞毒性测试表明该RBPH对Vero细胞无毒。此外，<50 kDa的RBPH对胃蛋白酶和胰蛋白酶的体外消化具有抗性。这些发现表明，含RBPH的ACE抑制肽可能比合成药物更安全，更健康，并且可以作为降低血压的有效食品补充剂。
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