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Differential regulation of jasmonic acid pathways in resistant (Calcutta 4) and susceptible (Williams) banana genotypes during the interaction with Pseudocercospora fijiensis (M. Morelet) Deighton.
Plant Pathology ( IF 2.3 ) Pub Date : 2020-03-12 , DOI: 10.1111/ppa.13165
Héctor Alejandro Rodriguez 1, 2 , William F. Hidalgo 3 , J. Danilo Sanchez 4 , Riya C. Menezes 5 , Bernd Schneider 6 , Rafael Eduardo Arango 1, 7 , Juan Gonzalo Morales 2
Affiliation  

One of the main biosecurity problems facing banana crops is black Sigatoka disease, caused by the fungus Pseudocercospora fijiensis. Disease control is achieved mainly by chemical sprays and generates more than 50% of the costs of production, with a trend to increase due to the rapid resistance that the fungus acquires to the main fungicide molecules. Thus, it is very important to acquire information on the molecular mechanisms of the plant–pathogen interactions in this pathosystem as a way to help design future control strategies. Hormonal plant responses of banana genotypes susceptible and resistant to P. fijiensis were identified and analysed in this work by transcriptomic (RNA‐Seq and RT‐qPCR) and metabolomic studies (ultraperformance liquid chromatography‐mass spectroscopy). Differentially expressed genes related to signal transduction and biosynthesis pathways of jasmonic acid (JA) and ethylene (ET) were identified in the resistant variety Calcutta 4 during the early stage of interaction with P. fijiensis. Metabolomic analysis corroborated the induction of metabolites related to JA and ET pathways during the first 72 hr post‐inoculation. Observed results are evidence that signalling via JA/ET could be key in the activation of defence response signals in the resistant variety Calcutta 4.

中文翻译:

在与 Pseudocercospora fijiensis (M. Morelet) Deighton 相互作用期间,抗性(加尔各答 4)和易感(威廉姆斯)香蕉基因型茉莉酸途径的差异调节。

香蕉作物面临的主要生物安全问题之一是由真菌 Pseudocercospora fijiensis 引起的黑色 Sigatoka 病。病害控制主要通过化学喷雾实现,占生产成本的 50% 以上,由于真菌对主要杀真菌剂分子的快速抗性,有增加的趋势。因此,获取有关该病理系统中植物-病原体相互作用的分子机制的信息非常重要,以帮助设计未来的控制策略。在这项工作中,通过转录组学(RNA-Seq 和 RT-qPCR)和代谢组学研究(超高效液相色谱-质谱)鉴定和分析了对 P. fijiensis 敏感和抗性的香蕉基因型的激素植物反应。在抗性品种加尔各答 4 号与斐济紫菜相互作用的早期,鉴定出与茉莉酸 (JA) 和乙烯 (ET) 信号转导和生物合成途径相关的差异表达基因。代谢组学分析证实了在接种后的前 72 小时内诱导了与 JA 和 ET 途径相关的代谢物。观察到的结果证明,通过 JA/ET 发出的信号可能是激活抗性品种加尔各答 4 中防御反应信号的关键。
更新日期:2020-03-12
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