BACKGROUND Blueberry (Vaccinium spp.) is a small berry with high economic value. Although cold storage can extend the storage time of blueberry to more than 60 days, it leads to chilling injury (CI) displaying as pedicle pits; and the samples of 0 °C-30 days was the critical point of CI. However, little is known about the mechanism and the molecular basis response to cold stress in blueberry have not been explained definitely. To comprehensively reveal the CI mechanisms in response to cold stress, we performed high-throughput RNA Seq analysis to investigate the gene regulation network in 0d (control) and 30d chilled blueberry. At the same time, the pitting and decay rate, electrolyte leakage (EL), malondialdehyde (MDA) proline content and GSH content were measured. RESULTS Two cDNA libraries from 0d (control) and 30d chilled samples were constructed and sequenced, generating a total of 35,060 unigenes with an N50 length of 1348 bp. Of these, 1852 were differentially expressed, with 1167 upregulated and 685 downregulated. Forty-five cold-induced transcription factor (TF) families containing 1023 TFs were identified. The DEGs indicated biological processes such as stress responses; cell wall metabolism; abscisic acid, gibberellin, membrane lipid, energy metabolism, cellular components, and molecular functions were significantly responsed to cold storage. The transcriptional level of 40 DEGs were verified by qRT-PCR. CONCLUSIONS The postharvest cold storage leads serious CI in blueberry, which substantially decreases the quality, storability and consumer acceptance. The MDA content, proline content, EL increased and the GSH content decreased in this chilled process. The biological processes such as stress responses, hormone metabolic processes were significantly affected by CI. Overall, the results obtained here are valuable for preventing CI under cold storage and could help to perfect the lack of the genetic information of non-model plant species.

中文翻译：

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采后蓝莓（Vaccinium corymbosum'Duke'）对冷胁迫的转录组分析。

背景技术蓝莓（Vaccinium spp。）是一种具有高经济价值的小浆果。尽管冷藏可以将蓝莓的保存时间延长至60天以上，但会导致冷冻伤害（CI）表现为椎弓根坑；0°C-30天的样品是CI的临界点。然而，关于机理和机理还知之甚少，蓝莓对冷胁迫的分子基础反应还没有得到明确的解释。为了全面揭示响应冷胁迫的CI机制，我们进行了高通量RNA Seq分析，以研究0d（对照）和30d冷藏蓝莓的基因调控网络。同时，测量了点蚀和衰减率，电解质渗漏（EL），丙二醛（MDA）脯氨酸含量和GSH含量。结果分别构建了0d（对照）和30d冷藏样品的两个cDNA文库并进行了测序，产生了35,060个单基因，N50长度为1348 bp。其中，有1852个差异表达，有1167个上调和685个下调。鉴定了包含1023个TF的45个冷诱导转录因子（TF）家族。DEGs表明了生物学过程，例如应激反应。细胞壁代谢 脱落酸，赤霉素，膜脂，能量代谢，细胞成分和分子功能对冷藏显着响应。通过qRT-PCR验证了40个DEGs的转录水平。结论收获后的冷藏导致蓝莓的严重CI，这大大降低了蓝莓的质量，耐贮藏性和消费者的接受度。MDA含量，脯氨酸含量，在该冷却过程中，EL升高而GSH含量降低。CI对诸如应激反应，激素代谢过程等生物学过程有显着影响。总体而言，此处获得的结果对于防止冷藏条件下的CI很有用，并且可以帮助完善非模型植物物种的遗传信息。