Extended Snake Venomics by Top-Down In-Source Decay: Investigating the Newly Discovered Anatolian Meadow Viper Subspecies, Vipera anatolica senliki.,Journal of Proteome Research

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Extended Snake Venomics by Top-Down In-Source Decay: Investigating the Newly Discovered Anatolian Meadow Viper Subspecies, Vipera anatolica senliki.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2020-02-19 , DOI: 10.1021/acs.jproteome.9b00869
Benjamin-Florian Hempel ₁ , Maik Damm ₁ , Mrinalini ₂ , Bayram Göçmen ₃ , Mert Karış ₃ , Ayse Nalbantsoy ₄ , R Manjunatha Kini _{2,

5} , Roderich D Süssmuth ₁

Affiliation

Herein we report on the venom proteome of Vipera anatolica senliki, a recently discovered and hitherto unexplored subspecies of the critically endangered Anatolian meadow viper endemic to the Antalya Province of Turkey. Integrative venomics, including venom gland transcriptomics as well as complementary bottom-up and top-down proteomic analyses, were applied to fully characterize the venom of V. a. senliki. Furthermore, the classical top-down venomics approach was extended to elucidate the venom proteome by an alternative in-source decay (ISD) proteomics workflow using the reducing matrix 1,5-diaminonaphthalene (1,5-DAN). Top-down ISD proteomics allows for disulfide bond counting as well as effective de novo sequencing-based identification of high molecular weight venom constituents, both of which are difficult to achieve by commonly established top-down approaches. Venom gland transcriptome analysis identified 96 toxin transcript annotations from 18 venom toxin families. Relative quantitative snake venomics revealed snake venom metalloproteinases (svMP, 42.9%) as the most abundant protein family, followed by several less dominant toxin families. Online mass profiling and top-down venomics provide a detailed insight into the venom proteome of V. a. senliki and facilitates a comparative analysis of venom variability for the closely related subspecies, V. a. anatolica.

中文翻译：

通过自上而下的源内衰减扩展了蛇的经济学：研究新发现的安纳托利亚草甸Vi蛇亚种per蛇亚种（Vipera anatolica senliki）。

在此，我们报告Vi蛇毒蛇毒蛋白质组，per蛇毒蛇是最近发现的，迄今尚未开发的土耳其安塔利亚省特有的极度濒危的安纳托利亚草甸vi蛇的亚种。包括毒液腺转录组学以及补充性的自下而上和自上而下的蛋白质组学分析在内的综合性病毒学被用来全面表征葡萄球菌的毒液。森利基。此外，经典的自上而下的经济学方法已扩展为通过使用还原性基质1,5-二氨基萘（1,5-DAN）的替代性源内衰减（ISD）蛋白质组学工作流程来阐明毒液蛋白质组。自上而下的ISD蛋白质组学可以对二硫键进行计数，并可以基于从头测序有效地鉴定高分子量毒液成分，这两种方法都很难通过通常建立的自上而下的方法来实现。毒腺转录组分析从18个毒毒素家族中鉴定出96个毒素转录本注释。相对定量的蛇病毒组学显示蛇毒金属蛋白酶（svMP，42.9％）是最丰富的蛋白质家族，其次是几个较弱的毒素家族。在线质量分析和自上而下的病毒学提供了对V. a。毒液蛋白质组的详细了解。senliki并有助于对密切相关的亚种V. a。的毒液变异性进行比较分析。安纳托利卡其次是几个不太重要的毒素家族。在线质量分析和自上而下的病毒学提供了对V. a。毒液蛋白质组的详细了解。senliki并有助于对密切相关的亚种V. a。的毒液变异性进行比较分析。安纳托利卡其次是几个不太重要的毒素家族。在线质量分析和自上而下的病毒学提供了对V. a。毒液蛋白质组的详细了解。senliki并有助于对密切相关的亚种V. a。的毒液变异性进行比较分析。安纳托利卡

更新日期：2020-02-19

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