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Novel Fusion Protein Consisting of Metallothionein, Cellulose Binding Module, and Superfolder GFP for Lead Removal from the Water Decoction of Traditional Chinese Medicine.
ACS Omega ( IF 3.7 ) Pub Date : 2020-02-03 , DOI: 10.1021/acsomega.9b03739 Qing Xiao 1 , Jing Han 1 , Chang Jiang 1 , Meng Luo 2 , Qingyi Zhang 3 , Zhaodong He 1 , Juan Hu 1, 3 , Guozeng Wang 2
ACS Omega ( IF 3.7 ) Pub Date : 2020-02-03 , DOI: 10.1021/acsomega.9b03739 Qing Xiao 1 , Jing Han 1 , Chang Jiang 1 , Meng Luo 2 , Qingyi Zhang 3 , Zhaodong He 1 , Juan Hu 1, 3 , Guozeng Wang 2
Affiliation
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Many methods have been used to detect heavy metals in herbal medicines, while few are developed to remove them. In this study, a novel genetically engineered fusion protein composed of metallothionein (MT), cellulose binding module (CBM), and superfolder GFP (sfGFP) was designed to remove heavy metals. MT, a kind of cysteine-rich protein, was used to chelate heavy metals with high specific affinity. The CBM facilitated the fusion protein MT-CBM-sfGFP binding to cellulose specifically, which made the purification and immobilization in one step. The sfGFP was used to detect the fusion protein MT-CBM-sfGFP easily during the process of expression and immobilization. The MT from Cancer pagurus (MTCap) and the CBM from Cellulomonas fimi (CBMCef) were used as an example and the fusion protein (MTCap-CBMCef-sfGFP) was expressed in Escherichia coli. Then, the cell lysates were mechanically mixed with cellulose to create biosorbent MTCap-CBMCef-sfGFP@cellulose. The efficiency of the biosorbent MTCap-CBMCef-sfGFP@cellulose for Pb2+ removal was evaluated using the water decoction of Honeysuckle as a model. Results suggested that MTCap-CBMCef-sfGFP@cellulose had high efficiency for Pb2+ removal from the water decoction of Honeysuckle without affecting its active ingredients. The low-cost, easy production, and high efficiency of the biosorbent enable it to have many applications in heavy metal removal from aqueous solutions of herbal medicines and food.
中文翻译：
由金属硫蛋白、纤维素结合模块和超级文件夹 GFP 组成的新型融合蛋白，用于去除中药水煎剂中的铅。
许多方法已被用来检测草药中的重金属，但很少有方法能够去除它们。在这项研究中，设计了一种由金属硫蛋白（MT）、纤维素结合模块（CBM）和超级文件夹GFP（sfGFP）组成的新型基因工程融合蛋白来去除重金属。 MT是一种富含半胱氨酸的蛋白质，用于以高特异性亲和力螯合重金属。 CBM促进融合蛋白MT-CBM-sfGFP与纤维素特异性结合，实现纯化和固定化一步完成。 sfGFP用于在表达和固定过程中轻松检测融合蛋白MT-CBM-sfGFP。以来自巨蟹座的MT（MTCap）和来自纤维单胞菌的CBM（CBMCef）为例，在大肠杆菌中表达融合蛋白（MTCap-CBMCef-sfGFP）。然后，将细胞裂解物与纤维素机械混合以产生生物吸附剂 MTCap-CBMCef-sfGFP@纤维素。以金银花水煎剂为模型，评价生物吸附剂MTCap-CBMCef-sfGFP@纤维素去除Pb2+的效率。结果表明，MTCap-CBMCef-sfGFP@纤维素对金银花水煎液中的 Pb2+ 具有高效去除效果，且不影响其活性成分。该生物吸附剂成本低廉、易于生产且效率高，使其在草药和食品水溶液中去除重金属方面具有广泛的应用。
更新日期：2020-02-18
中文翻译：
由金属硫蛋白、纤维素结合模块和超级文件夹 GFP 组成的新型融合蛋白，用于去除中药水煎剂中的铅。
许多方法已被用来检测草药中的重金属，但很少有方法能够去除它们。在这项研究中，设计了一种由金属硫蛋白（MT）、纤维素结合模块（CBM）和超级文件夹GFP（sfGFP）组成的新型基因工程融合蛋白来去除重金属。 MT是一种富含半胱氨酸的蛋白质，用于以高特异性亲和力螯合重金属。 CBM促进融合蛋白MT-CBM-sfGFP与纤维素特异性结合，实现纯化和固定化一步完成。 sfGFP用于在表达和固定过程中轻松检测融合蛋白MT-CBM-sfGFP。以来自巨蟹座的MT（MTCap）和来自纤维单胞菌的CBM（CBMCef）为例，在大肠杆菌中表达融合蛋白（MTCap-CBMCef-sfGFP）。然后，将细胞裂解物与纤维素机械混合以产生生物吸附剂 MTCap-CBMCef-sfGFP@纤维素。以金银花水煎剂为模型，评价生物吸附剂MTCap-CBMCef-sfGFP@纤维素去除Pb2+的效率。结果表明，MTCap-CBMCef-sfGFP@纤维素对金银花水煎液中的 Pb2+ 具有高效去除效果，且不影响其活性成分。该生物吸附剂成本低廉、易于生产且效率高，使其在草药和食品水溶液中去除重金属方面具有广泛的应用。
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