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GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-02-18 , DOI: 10.1111/jipb.12919 Huihui Zhang 1, 2, 3 , Menglong Wang 1, 2 , Yiqi Li 1, 2 , Wei Yan 1, 2 , Zhenyi Chang 1, 2 , Haoling Ni 1 , Zhufeng Chen 2 , Jianxin Wu 1 , Chunjue Xu 2 , Xing Wang Deng 2, 4 , Xiaoyan Tang 1, 2
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-02-18 , DOI: 10.1111/jipb.12919 Huihui Zhang 1, 2, 3 , Menglong Wang 1, 2 , Yiqi Li 1, 2 , Wei Yan 1, 2 , Zhenyi Chang 1, 2 , Haoling Ni 1 , Zhufeng Chen 2 , Jianxin Wu 1 , Chunjue Xu 2 , Xing Wang Deng 2, 4 , Xiaoyan Tang 1, 2
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Pollen exine contains complex biopolymers of aliphatic lipids and phenolics. Abnormal development of pollen exine often leads to plant sterility. Molecular mechanisms regulating exine formation have been studied extensively but remain ambiguous. Here we report the analyses of three GDSL esterase/lipase protein genes, OsGELP34, OsGELP110, and OsGELP115, for rice exine formation. OsGELP34 was identified by cloning of a male sterile mutant gene. OsGELP34 encodes an endoplasmic reticulum protein and was mainly expressed in anthers during pollen exine formation. osgelp34 mutant displayed abnormal exine and altered expression of a number of key genes required for pollen development. OsGELP110 was previously identified as a gene differentially expressed in meiotic anthers. OsGELP110 was most homologous to OsGELP115, and the two genes showed similar gene expression patterns. Both OsGELP110 and OsGELP115 proteins were localized in peroxisomes. Individual knockout of OsGELP110 and OsGELP115 did not affect the plant fertility, but double knockout of both genes altered the exine structure and rendered the plant male sterile. OsGELP34 is distant from OsGELP110 and OsGELP115 in sequence, and osgelp34 and osgelp110/osgelp115 mutants were different in anther morphology despite both were male sterile. These results suggested that OsGELP34 and OsGELP110/OsGELP115 catalyze different compounds for pollen exine development.
中文翻译：
GDSL酯酶/脂肪酶OsGELP34和OsGELP110 / OsGELP115对水稻花粉发育至关重要。
花粉外膜含有脂族脂质和酚类的复杂生物聚合物。花粉外壁发育异常常导致植物不育。调节外壁形成的分子机制已被广泛研究，但仍不清楚。在这里，我们报告了三个GDSL酯酶/脂肪酶蛋白基因OsGELP34，OsGELP110和OsGELP115的分析，这些基因用于水稻外壁形成。通过克隆雄性不育突变基因鉴定出OsGELP34。OsGELP34编码内质网蛋白，主要在花粉外壁形成过程中的花药中表达。osgelp34突变体显示出异常的外壁，并改变了花粉发育所需的许多关键基因的表达。OsGELP110先前被鉴定为在减数分裂花药中差异表达的基因。OsGELP110是最同源的OsGELP115，并且两个基因表现出类似的基因表达模式。OsGELP110和OsGELP115蛋白都位于过氧化物酶体中。单独敲除OsGELP110和OsGELP115不会影响植物的育性，但是两次敲除两个基因都会改变外在结构，并使植物雄性不育。OsGELP34依次远离OsGELP110和OsGELP115，以及osgelp34和osgelp110 / osgelp115尽管两个突变体均为雄性不育，但它们的花药形态却不同。这些结果表明，OsGELP34和OsGELP110 / OsGELP115催化花粉外壁发育的不同化合物。
更新日期：2020-02-18
中文翻译：
GDSL酯酶/脂肪酶OsGELP34和OsGELP110 / OsGELP115对水稻花粉发育至关重要。
花粉外膜含有脂族脂质和酚类的复杂生物聚合物。花粉外壁发育异常常导致植物不育。调节外壁形成的分子机制已被广泛研究，但仍不清楚。在这里，我们报告了三个GDSL酯酶/脂肪酶蛋白基因OsGELP34，OsGELP110和OsGELP115的分析，这些基因用于水稻外壁形成。通过克隆雄性不育突变基因鉴定出OsGELP34。OsGELP34编码内质网蛋白，主要在花粉外壁形成过程中的花药中表达。osgelp34突变体显示出异常的外壁，并改变了花粉发育所需的许多关键基因的表达。OsGELP110先前被鉴定为在减数分裂花药中差异表达的基因。OsGELP110是最同源的OsGELP115，并且两个基因表现出类似的基因表达模式。OsGELP110和OsGELP115蛋白都位于过氧化物酶体中。单独敲除OsGELP110和OsGELP115不会影响植物的育性，但是两次敲除两个基因都会改变外在结构，并使植物雄性不育。OsGELP34依次远离OsGELP110和OsGELP115，以及osgelp34和osgelp110 / osgelp115尽管两个突变体均为雄性不育，但它们的花药形态却不同。这些结果表明，OsGELP34和OsGELP110 / OsGELP115催化花粉外壁发育的不同化合物。
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