Real‐Time Imaging Tracking of Engineered Macrophages as Ultrasound‐Triggered Cell Bombs for Cancer Treatment,Advanced Functional Materials

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Real‐Time Imaging Tracking of Engineered Macrophages as Ultrasound‐Triggered Cell Bombs for Cancer Treatment
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2020-02-16 , DOI: 10.1002/adfm.201910304
Zhili Xu _{1,

2} , Hongmei Liu _{1,

2} , Hao Tian ₁ , Fei Yan _{2,

3}

Affiliation

Cell‐based drug delivery systems are a promising platform for tumor‐targeted therapy due to their high drug‐loading capacities and inherent tumor‐homing abilities. However, the real‐time tracking of these carrier cells and controlled release of the encapsulated drugs are still challenging. Here, ultrasound‐activatable cell bombs are developed by encapsulating doxorubicin (DOX) and phase transformable perfluoropentane (PFP) into hollow mesoporous organosilica nanoparticles (HMONs) to prepare DOX/PFP‐loaded HMONs (DPH), followed by internalization into macrophages (RAW 264.7 cells). The resulting cell bombs (DPH‐RAWs) can maintain viability and actively home to the tumor. Especially, their migration can be tracked in real time using ultrasound due to the vaporization of a small portion of PFP during cell incubation at 37 °C. After accumulation at the tumor site, the further vaporization of remaining PFP can be triggered by a short‐pulsed high intensity focused ultrasound (HIFU) sonication, resulting in the generation of several large microbubbles, which destroys DPH‐RAWs and allows drug release out of these cells. The DPH‐RAWs combined with short‐pulsed HIFU sonication significantly inhibit tumor growth and prolong survival of tumor‐bearing mice. In conclusion, this study provides a new approach to cell‐based drug delivery systems for real‐time tracking of their migration and targeted cancer treatment.

中文翻译：

工程巨噬细胞作为超声触发的细胞炸弹的实时成像跟踪，用于癌症治疗

基于细胞的药物输送系统具有很高的载药量和固有的肿瘤归巢能力，因此是靶向肿瘤治疗的有前途的平台。但是，这些载体细胞的实时跟踪和封装药物的控制释放仍然具有挑战性。在这里，通过将阿霉素（DOX）和可相变的全氟戊烷（PFP）封装到空心介孔有机硅纳米粒子（HMON）中以制备DOX / PFP负载的HMONs（DPH），然后内化为巨噬细胞，来开发可超声激活的细胞炸弹。细胞）。产生的细胞炸弹（DPH-RAWs）可以维持生存力并活跃地成为肿瘤的宿主。特别是，由于在37°C的细胞温育过程中一小部分PFP的蒸发，可以使用超声波实时跟踪其迁移。在肿瘤部位积聚后，短脉冲高强度聚焦超声（HIFU）超声可触发剩余PFP的进一步汽化，从而导致产生多个大的微气泡，从而破坏DPH-RAWs并使药物释放出这些细胞。DPH-RAWs结合短脉冲HIFU超声处理可显着抑制肿瘤生长并延长荷瘤小鼠的生存期。总之，本研究为基于细胞的药物输送系统提供了一种新方法，用于实时跟踪其迁移和靶向癌症治疗。DPH-RAWs结合短脉冲HIFU超声处理可显着抑制肿瘤生长并延长荷瘤小鼠的生存期。总之，本研究为基于细胞的药物输送系统提供了一种新方法，用于实时跟踪其迁移和靶向癌症治疗。DPH-RAWs结合短脉冲HIFU超声处理可显着抑制肿瘤生长并延长荷瘤小鼠的生存期。总之，本研究为基于细胞的药物输送系统提供了一种新方法，用于实时跟踪其迁移和靶向癌症治疗。

更新日期：2020-04-06

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