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A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2020-02-16 , DOI: 10.1002/adfm.201907297 Sharat Chandra Barman 1 , Md. Abu Zahed 1 , Md. Sharifuzzaman 1 , Seok Gyu Ko 1 , Hyosang Yoon 1 , Joong San Nah 1 , Xing Xuan 1 , Jae Yeong Park 1
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2020-02-16 , DOI: 10.1002/adfm.201907297 Sharat Chandra Barman 1 , Md. Abu Zahed 1 , Md. Sharifuzzaman 1 , Seok Gyu Ko 1 , Hyosang Yoon 1 , Joong San Nah 1 , Xing Xuan 1 , Jae Yeong Park 1
Affiliation
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Current immunosensors have an insufficient number of binding sites for the recognition of biomolecules, which leads to false positive or negative results. In this research, a facile, cost‐effective, disposable, and highly selective electrochemical immunosensing platform is developed based on cationic polyelectrolyte polyallylamine (PAAMI) anchored laser‐ablated graphene (LAG). Here, for the first time, PAAMI is introduced to stabilize LAG flakes, while retaining the intrinsic thermal and electronic properties of the substrate by noncovalent π–π interaction and electrostatic physical absorption. The sensing platform offers a suitable number of anchoring sites for the immobilized antibodies by providing NH2 functional groups. The proper grafting of PAAMI is confirmed through X‐ray photoelectron spectroscopy and Raman spectroscopy. The immunosensing platform is applied to detect immunoglobulin (IgG) biomarkers as a proof of concept. Under optimized conditions, the sensing platform exhibits a linear range of 0.012–15 and 15–352 ng mL−1 with a limit of detection of 6 pg mL−1 for IgG detection with high selectivity. Based on the analysis, the developed immunosensing platform can be used for point‐of‐care detection of IgG in clinical diagnostic centers. Furthermore, the developed strategy is well suited for the detection of other cancer biomarkers after immobilizing the relevant antibodies.
中文翻译：
聚烯丙胺固定的富含胺的激光烧蚀石墨烯平台，用于简便且高度选择性的电化学IgG生物标志物检测
当前的免疫传感器的结合位点数量不足以识别生物分子，这导致假阳性或阴性结果。在这项研究中，开发了一种基于阳离子聚电解质聚烯丙基胺（PAAMI）锚定激光烧蚀石墨烯（LAG）的简便，经济高效，一次性且高度选择性的电化学免疫传感平台。在这里，首次引入PAAMI来稳定LAG薄片，同时通过非共价π-π相互作用和静电物理吸收保留基材的固有热和电子性能。感测平台提供锚定为固定的抗体位点的合适数量的通过提供 NH 2功能组。X射线光电子能谱和拉曼光谱证实了PAAMI的正确接枝。免疫传感平台可用于检测免疫球蛋白（IgG）生物标志物，作为概念证明。在优化的条件下，传感平台的线性范围为0.012–15和15–352 ng mL -1，对于高选择性的IgG检测，检测限为6 pg mL -1。根据分析，开发的免疫传感平台可用于临床诊断中心的IgG即时点检测。此外，在固定相关抗体后，开发的策略非常适合于检测其他癌症生物标记。
更新日期：2020-04-06
中文翻译：
聚烯丙胺固定的富含胺的激光烧蚀石墨烯平台，用于简便且高度选择性的电化学IgG生物标志物检测
当前的免疫传感器的结合位点数量不足以识别生物分子，这导致假阳性或阴性结果。在这项研究中，开发了一种基于阳离子聚电解质聚烯丙基胺（PAAMI）锚定激光烧蚀石墨烯（LAG）的简便，经济高效，一次性且高度选择性的电化学免疫传感平台。在这里，首次引入PAAMI来稳定LAG薄片，同时通过非共价π-π相互作用和静电物理吸收保留基材的固有热和电子性能。感测平台提供锚定为固定的抗体位点的合适数量的通过提供 NH 2功能组。X射线光电子能谱和拉曼光谱证实了PAAMI的正确接枝。免疫传感平台可用于检测免疫球蛋白（IgG）生物标志物，作为概念证明。在优化的条件下，传感平台的线性范围为0.012–15和15–352 ng mL -1，对于高选择性的IgG检测，检测限为6 pg mL -1。根据分析，开发的免疫传感平台可用于临床诊断中心的IgG即时点检测。此外，在固定相关抗体后，开发的策略非常适合于检测其他癌症生物标记。
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