Controlled division of cell-sized vesicles by low densities of membrane-bound proteins.,Nature Communications

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Controlled division of cell-sized vesicles by low densities of membrane-bound proteins.
Nature Communications ( IF 14.7 ) Pub Date : 2020-02-14 , DOI: 10.1038/s41467-020-14696-0
Jan Steinkühler ₁ , Roland L Knorr ₁ , Ziliang Zhao ₁ , Tripta Bhatia ₁ , Solveig M Bartelt ₂ , Seraphine Wegner ₂ , Rumiana Dimova ₁ , Reinhard Lipowsky ₁

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The proliferation of life on earth is based on the ability of single cells to divide into two daughter cells. During cell division, the plasma membrane undergoes a series of morphological transformations which ultimately lead to membrane fission. Here, we show that analogous remodeling processes can be induced by low densities of proteins bound to the membranes of cell-sized lipid vesicles. Using His-tagged fluorescent proteins, we are able to precisely control the spontaneous curvature of the vesicle membranes. By fine-tuning this curvature, we obtain dumbbell-shaped vesicles with closed membrane necks as well as neck fission and complete vesicle division. Our results demonstrate that the spontaneous curvature generates constriction forces around the membrane necks and that these forces can easily cover the force range found in vivo. Our approach involves only one species of membrane-bound proteins at low densities, thereby providing a simple and extendible module for bottom-up synthetic biology.

中文翻译：

通过膜结合蛋白的低密度控制细胞大小的囊泡的分裂。

地球上生命的增殖是基于单个细胞分裂成两个子细胞的能力。在细胞分裂期间，质膜经历一系列形态学转变，最终导致膜裂变。在这里，我们表明可以通过与细胞大小的脂质囊泡膜结合的低密度蛋白质诱导类似的重塑过程。使用带有His标签的荧光蛋白，我们能够精确控制囊泡膜的自发曲率。通过微调此曲率，我们获得了具有闭合的膜颈部，裂口和完整囊泡分裂的哑铃形囊泡。我们的结果表明，自发曲率会在膜颈部周围产生收缩力，并且这些力可以轻松覆盖体内发现的力范围。

更新日期：2020-02-17

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