In the present work, a novel biocompatible scaffold was fabricated for the DNA aptamer immobilization. For the first time, amino-functionalized dendritic fibrous nanosilica (KCC-1-nPr-NH2 ) and gold nanoparticle supported by chitosan (AuNPs-CS) were synthesized and electrodeposited successfully on the surface of the glassy carbon electrode by chronoamperometry technique. Unique oligonucleotide of aflatoxin M1 (5'-ATC CGT CAC ACC TGC TCT GAC GCT GGG GTC GAC CCG GAG AAA TGC ATT CCC CTG TGG TGT TGG CTC CCG TAT) labeled by toluidine blue was immobilization on the prepared interface. Hence, a novel aptamer-based bioassay was formed for highly sensitive quantitation of AFM1 using cyclic voltammetry and differential plus voltammetry. The structure and morphology of GQDs-CS/KCC-1-nPr-NH2 were investigated by Fourier-transform infrared spectroscopy, X-ray diffraction, atomic force, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The achieved low limit of quantification of apta-assay for detection of AFM1 was 10fM. Also, calibration curve was linear from 0.1μM to 10fM in real samples. The proposed apta-assay has acceptable long-term stability. Designed aptasensor has a lot of remarkable advantages including excellent selectivity, sensitivity, and stability that could be used as facile bio-device for the determination of AFM1 in milk samples.

中文翻译：

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基于开启方法使用适体与 DNFS 结合对实际样品中的黄曲霉毒素 M1 进行特异性监测：一种新型生物传感器。

在目前的工作中，为 DNA 适配体固定制造了一种新型的生物相容性支架。首次合成了氨基功能化的树枝状纤维状纳米二氧化硅（KCC-1-nPr-NH2）和壳聚糖负载的金纳米颗粒（AuNPs-CS），并通过计时电流法成功地将其电沉积在玻碳电极表面。用甲苯胺蓝标记的黄曲霉毒素M1（5'-ATC CGT CAC ACC TGC TCT GAC GCT GGG GTC GAC CCG GAG AAA TGC ATT CCC CTG TGG TGT TGG CTC CCG TAT）的独特寡核苷酸固定在制备的界面上。因此，形成了一种新的基于适体的生物测定法，用于使用循环伏安法和微分加伏安法对 AFM1 进行高灵敏度定量。通过傅里叶变换红外光谱研究了 GQDs-CS/KCC-1-nPr-NH2 的结构和形貌，X 射线衍射、原子力、扫描电子显微镜和能量色散 X 射线光谱。用于检测 AFM1 的适体测定的量化下限为 10fM。此外，校准曲线在实际样品中从 0.1μM 到 10fM 呈线性。提议的适体测定具有可接受的长期稳定性。所设计的适体传感器具有许多显着的优点，包括优异的选择性、灵敏度和稳定性，可用作测定牛奶样品中 AFM1 的简便生物装置。