The Andean native potato is an important food security crop and constitutes a gene pool for potato-breeding. To establish the current state of the native potatoes diversity in the influence zone of the Camisea Gas Project, North Ayacucho, Peru, landraces (n = 144) were collected. Three sampling populations (Anco, Chungui and Ticllas) were resolved using 10 simple sequence repeats (SSRs) markers that amplified 67 polymorphic alleles. Principal component, correspondence and cluster analysis revealed a minimum set of six SSRs to achieve DNA fingerprinting and cost-effective genetic diversity analysis. Analysis of molecular variance (AMOVA), average fixation index (F_ST = 0.279), and genetic distance index (S_B) indicated that the genetic diversity of the native potatoes is high at the intra- and inter-population levels, and each of the three sampling populations constituted closed populations. The three populations were genetically distinct and contained unique genotypes and exclusive alleles. Misidentified native potatoes (10.41%) were found.

中文翻译：

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基于简单重复序列的遗传多样性和秘鲁阿亚库乔北部Camisea天然气项目影响区的农用本地马铃薯本地品种的分子变异分析

安第斯本地马铃薯是重要的粮食安全作物，并且构成了马铃薯育种的基因库。为了确定秘鲁北部阿亚库乔的Camisea天然气项目影响区的本地马铃薯多样性的现状 ，收集了地方品种（n = 144）。使用10个简单序列重复（SSR）标记解析了三个抽样人群（Anco，Chungui和Ticllas），扩增了67个多态性等位基因。主要成分，对应关系和聚类分析揭示了至少六个SSR的集合，以实现DNA指纹和具有成本效益的遗传多样性分析。分析分子变异（AMOVA），平均固着指数（F _ST  = 0.279）和遗传距离指数（S _B）表明，本地马铃薯的遗传多样性在种群内和种群间水平较高，并且三个抽样种群中的每一个均构成封闭种群。这三个种群在遗传上是不同的，并且包含独特的基因型和排他的等位基因。发现了误认的原生马铃薯（10.41％）。