The current studies provide insights into how predictions based on results of physicochemical and in vitro tests are consistent with the results of in vivo studies. The new biomicroconcrete type materials were obtained by mixing the solid phase, composed of hybrid hydroxyapatite/chitosan granules and highly reactive α‐tricalcium phosphate powder, used as the setting agent. This approach guaranteed a good adhesion of the continuous cement phase to the surface of granules. It has been demonstrated that developed biomicroconcretes are surgically handy, possessed favorable physicochemical and biological properties and can be considered as effective bone implant material. The hierarchical porosity and compressive strength (2–6 MPa) similar to cancellous bone made them suitable for low‐load bearing applications. Despite the fact that final setting times of biomicroconcretes were longer than recommended in the literature (i.e., exceeded 15 min), their short cohesion time allows for a successful implantation in a rabbit femoral defect model. Histological analysis and Raman studies revealed newly formed bone tissues around the sides of implanted materials. Furthermore, the process of neovascularization and reconstruction of the bone tissue, as well as a reverse scaffolding process, was visible. No signs of inflammation or adverse tissue reactions were observed during the experiment.

中文翻译：

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基于α-磷酸三钙和混合羟基磷灰石/壳聚糖颗粒和海藻酸钠的生物微混凝土的体内行为。

当前的研究提供了有关基于物理化学和体外测试结果的预测如何与体内研究结果一致的见解。通过混合由混合羟基磷灰石/壳聚糖颗粒和高活性α-磷酸三钙粉末组成的固相作为固化剂，获得了新型生物微混凝土类型材料。这种方法保证了连续水泥相与颗粒表面的良好粘附。已经证明，开发的生物微混凝土手术方便，具有良好的理化和生物学特性，可以被认为是有效的骨植入材料。类似于松质骨的分级孔隙率和抗压强度 (2-6 MPa) 使它们适用于低负载应用。尽管生物微混凝土的最终凝结时间比文献中推荐的要长（即超过 15 分钟），但它们的短内聚时间允许在兔股骨缺损模型中成功植入。组织学分析和拉曼研究揭示了植入材料两侧新形成的骨组织。此外，骨组织的新血管形成和重建过程以及反向支架过程是可见的。在实验过程中没有观察到炎症或不良组织反应的迹象。组织学分析和拉曼研究揭示了植入材料两侧新形成的骨组织。此外，骨组织的新血管形成和重建过程以及反向支架过程是可见的。在实验过程中没有观察到炎症或不良组织反应的迹象。组织学分析和拉曼研究揭示了植入材料两侧新形成的骨组织。此外，骨组织的新血管形成和重建过程以及反向支架过程是可见的。在实验过程中没有观察到炎症或不良组织反应的迹象。