Centrosomes are essential organelles with functions in microtubule organization that duplicate once per cell cycle. The first step of centrosome duplication is the daughter centriole formation followed by the pericentriolar material recruitment to this centriole. This maturation step was termed centriole-to-centrosome conversion. It was proposed that CEP295-dependent recruitment of pericentriolar proteins drives centriole conversion. Here we show, based on the analysis of proteins that promote centriole biogenesis, that the developing centriole structure helps drive centriole conversion. Depletion of the luminal centriole protein CEP44 that binds to the A-microtubules and interacts with POC1B affecting centriole structure and centriole conversion, despite CEP295 binding to centrioles. Impairment of POC1B, TUBE1 or TUBD1, which disturbs integrity of centriole microtubules, also prevents centriole-to-centrosome conversion. We propose that the CEP295, CEP44, POC1B, TUBE1 and TUBD1 centriole biogenesis pathway that functions in the centriole lumen and on the cytoplasmic side is essential for the centriole-to-centrosome conversion.

中文翻译：

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CEP44确保形成真正的中心体壁，这是中心体向中心体转化的要求。

中心体是在微管组织中具有功能的必需细胞器，每个细胞周期复制一次。中心体复制的第一步是形成子中心体，然后将中心体周围物质募集到该中心体。该成熟步骤被称为中心到中心体的转化。有人提出，依赖于CEP295的中心粒周围蛋白募集可以驱动中心粒转化。在这里，我们基于对促进中心粒生物发生的蛋白质的分析表明，正在发展的中心粒结构有助于驱动中心粒转化。尽管CEP295与中心粒结合，但与A微管结合并与POC1B相互作用的管腔中心粒蛋白CEP44耗竭，影响中心粒结构和中心粒转化。POC1B，TUBE1或TUBD1的损伤，这干扰了中心微管的完整性，也阻止了中心向中心体的转化。我们建议，CEP295，CEP44，POC1B，TUBE1和TUBD1中心体生物发生途径在中心体腔和细胞质侧起作用，对于中心体向中心体的转化至关重要。