In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila.,Nature Communications

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In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila.
Nature Communications ( IF 14.7 ) Pub Date : 2020-02-14 , DOI: 10.1038/s41467-020-14651-z
Rodrigo G Arzate-Mejía ₁ , Angel Josué Cerecedo-Castillo ₁ , Georgina Guerrero ₁ , Mayra Furlan-Magaril ₁ , Félix Recillas-Targa ₁

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Chromosomes are organized into high-frequency chromatin interaction domains called topologically associating domains (TADs), which are separated from each other by domain boundaries. The molecular mechanisms responsible for TAD formation are not yet fully understood. In Drosophila, it has been proposed that transcription is fundamental for TAD organization while the participation of genetic sequences bound by architectural proteins (APs) remains controversial. Here, we investigate the contribution of domain boundaries to TAD organization and the regulation of gene expression at the Notch gene locus in Drosophila. We find that deletion of domain boundaries results in TAD fusion and long-range topological defects that are accompanied by loss of APs and RNA Pol II chromatin binding as well as defects in transcription. Together, our results provide compelling evidence of the contribution of discrete genetic sequences bound by APs and RNA Pol II in the partition of the genome into TADs and in the regulation of gene expression in Drosophila.

中文翻译：

域边界的原位解剖影响果蝇中的基因组拓扑和基因转录。

染色体被组织为高频染色质相互作用域，称为拓扑关联域（TAD），它们之间通过域边界相互分隔。尚未完全了解造成TAD形成的分子机制。在果蝇中，已经提出转录是TAD组织的基础，而由建筑蛋白（AP）结合的遗传序列的参与仍存在争议。在这里，我们调查果蝇边界域对TAD组织的贡献和Notch基因位点的基因表达调控。我们发现删除域边界会导致TAD融合和远距离拓扑缺陷，并伴随AP丢失和RNA Pol II染色质结合以及转录缺陷。一起，

更新日期：2020-02-14

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