To study differences in SRB1 gene expression between Candida albicans fluconazole-resistant strains and fluconazole-sensitive strains, and to identify new antifungal drug treatment targets. We studied 30 fluconazole-resistant and 47 fluconazole-sensitive C. albicans strains. The strains were routinely cultured, and total RNA was extracted, reverse transcribed into cDNA and analyzed with real-time PCR amplification with 18S rRNA used as an internal reference gene. The expression levels of the two groups were analyzed in Light Cycler system software version 3.0, and independent Student's t test was performed in SPSS 19.0 statistical software. P < 0.05 was considered to indicate a statistical difference. DNAMAN multiple sequence alignment analysis was used to randomly analyze SBR1 related sequences of four resistant strains and four sensitive strains. An evolutionary tree was constructed with the maximum likelihood method in Mega6.0 software. The mean SRB1 gene expression in the drug-resistant group was 0.75138 × 10-3, and that in the sensitive group was 1.6664 × 10-3. Independent Student's t test indicated a statistically significant difference (T = -3.972, P = 0.000, P < 0.05). DNAMAN multiple sequence alignment analysis showed that the sequence identity of the CDS in the eight strains was 75.17%, and that of sequences 1000 bp upstream of the CDS was 96.35%. Cluster analysis showed that the CDS and sequences 1000 bp upstream of the CDS showed no significant differences between groups. At the mRNA level, the SRB1 gene expression in fluconazole-resistant C. albicans was lower than that in fluconazole-sensitive strains, thus suggesting that the gene may be associated with drug resistance and that the regulatory mechanism leading to this difference is complex.

中文翻译：

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SRB1基因在耐氟康唑的白色念珠菌易感菌株中的差异表达分析。

研究白色念珠菌耐氟康唑菌株和耐氟康唑敏感性菌株SRB1基因表达的差异，并确定新的抗真菌药物治疗靶标。我们研究了30氟康唑耐药和47氟康唑敏感性白色念珠菌菌株。常规培养菌株，提取总RNA，反转录为cDNA，并以18S rRNA作为内部参考基因进行实时PCR扩增分析。在Light Cycler系统软件版本3.0中分析两组的表达水平，并在SPSS 19.0统计软件中进行独立的Student t检验。P <0.05被认为表明有统计学差异。DNAMAN多序列比对分析用于随机分析四种抗性菌株和四种敏感菌株的SBR1相关序列。在Mega6.0软件中使用最大似然法构建了进化树。耐药组中SRB1基因的平均表达为0.75138×10-3，而敏感组中的SRB1基因平均表达为1.6664×10-3。独立学生t检验显示出统计学上的显着差异（T = -3.972，P = 0.000，P <0.05）。DNAMAN多序列比对分析表明，八株菌株中CDS的序列同一性为75.17％，CDS上游1000 bp的序列为96.35％。聚类分析表明，CDS和CDS上游1000 bp的序列在组之间没有显着差异。在mRNA水平上