Cell isolation from blood is an important process for diagnosing immune diseases. There are still demands for a user-friendly approach to achieve high cell extraction efficiency and purity of a target immune cell subtype for more promising diagnosis and monitoring. For selective immune cell isolation, we developed a microstructured device, which consists of antibody-coated micropillars and micro-sieve arrays, for isolating a target immune cell subtype from bovine blood samples. The focusing micropillars can guide immune cells flowing to the subsequent micro-sieves based on deterministic lateral shifts of the cells. The arrangement of these microstructures is characterized and configured for the maximal cell capture rate. Surface modification with a selected antibody offers selective cell capture in the micro-sieves based on the antigen-antibody reaction. We prepare a cell mixture of human CD14-expressing leukemia cells (THP-1) and epithelial cells (MDA-MB-231) in diluted blood to characterize the cell isolation operation, with a selective cell isolation yield of >80%, cell purity of ∼100% and cell viability of >93%. Together, this microstructured device strategy can achieve high-yield selective isolation of immune cells from blood samples and support downstream genetic and biochemical cell analyses, contributing to the medical diagnosis of a broad range of immune diseases.

中文翻译：

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用于选择性分离血液中免疫细胞的抗体涂层微结构。

从血液中分离细胞是诊断免疫疾病的重要过程。仍然需要一种用户友好的方法来实现目标免疫细胞亚型的高细胞提取效率和纯度，从而更有希望地进行诊断和监测。对于选择性免疫细胞分离，我们开发了一种微结构装置，该装置由涂有抗体的微柱和微筛阵列组成，用于从牛血样中分离目标免疫细胞亚型。聚焦微柱可以根据细胞的确定性横向移动引导免疫细胞流向后续的微筛。这些微结构的排列被表征和配置用于最大的细胞捕获率。用选定的抗体进行表面修饰可根据抗原-抗体反应在微筛中提供选择性的细胞捕获。我们在稀释的血液中制备了表达人类 CD14 的白血病细胞 (THP-1) 和上皮细胞 (MDA-MB-231) 的细胞混合物，以表征细胞分离操作，选择性细胞分离产量 > 80%，细胞纯度〜100％和细胞活力> 93％。总之，这种微结构设备策略可以实现从血液样本中高产率的选择性分离免疫细胞，并支持下游的遗传和生化细胞分析，有助于广泛免疫疾病的医学诊断。选择性细胞分离产率>80%，细胞纯度~100%，细胞活力>93%。总之，这种微结构设备策略可以实现从血液样本中高产率的选择性分离免疫细胞，并支持下游的遗传和生化细胞分析，有助于广泛免疫疾病的医学诊断。选择性细胞分离产率>80%，细胞纯度~100%，细胞活力>93%。总之，这种微结构设备策略可以实现从血液样本中高产率的选择性分离免疫细胞，并支持下游的遗传和生化细胞分析，有助于广泛免疫疾病的医学诊断。