RHS-elements function as type II toxin-antitoxin modules that regulate intra-macrophage replication of Salmonella Typhimurium.,PLOS Genetics

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RHS-elements function as type II toxin-antitoxin modules that regulate intra-macrophage replication of Salmonella Typhimurium.
PLOS Genetics ( IF 4.0 ) Pub Date : 2020-02-13 , DOI: 10.1371/journal.pgen.1008607
Magnus Stårsta ₁ , Disa L Hammarlöf ₁ , Marcus Wäneskog ₁ , Susan Schlegel ₁ , Feifei Xu ₁ , Arvid Heden Gynnå ₁ , Malin Borg ₁ , Sten Herschend ₁ , Sanna Koskiniemi ₁

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RHS elements are components of conserved toxin-delivery systems, wide-spread within the bacterial kingdom and some of the most positively selected genes known. However, very little is known about how Rhs toxins affect bacterial biology. Salmonella Typhimurium contains a full-length rhs gene and an adjacent orphan rhs gene, which lacks the conserved delivery part of the Rhs protein. Here we show that, in addition to the conventional delivery, Rhs toxin-antitoxin pairs encode for functional type-II toxin-antitoxin (TA) loci that regulate S. Typhimurium proliferation within macrophages. Mutant S. Typhimurium cells lacking both Rhs toxins proliferate 2-times better within macrophages, mainly because of an increased growth rate. Thus, in addition to providing strong positive selection for the rhs loci under conditions when there is little or no toxin delivery, internal expression of the toxin-antitoxin system regulates growth in the stressful environment found inside macrophages.

中文翻译：

RHS元素作为II型毒素-抗毒素模块，可调节伤寒沙门氏菌在巨噬细胞内的复制。

RHS元素是保守的毒素传递系统的组成部分，在细菌界广泛传播，并且是一些已知的最积极选择的基因。但是，人们对Rhs毒素如何影响细菌生物学知之甚少。鼠伤寒沙门氏菌包含一个全长的rhs基因和一个相邻的孤儿rhs基因，后者缺少Rhs蛋白的保守传递部分。在这里，我们显示，除常规递送外，Rhs毒素-抗毒素对还编码功能性II型毒素-抗毒素（TA）基因座，可调节巨噬细胞中鼠伤寒沙门氏菌的增殖。缺少这两种Rhs毒素的突变型鼠伤寒沙门氏菌细胞在巨噬细胞中的增殖能力提高了2倍，这主要是由于生长速率的提高。从而，

更新日期：2020-03-05

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