Bone-strengthening effects and safety of compound peptides from skin of Chiloscyllium plagiosum and Mustelus griseus.,Food Science & Nutrition

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Bone-strengthening effects and safety of compound peptides from skin of Chiloscyllium plagiosum and Mustelus griseus.
Food Science & Nutrition ( IF 3.5 ) Pub Date : 2020-02-13 , DOI: 10.1002/fsn3.1438
Xin-Heng Xu ₁ , Peng-Fei Lv ₁ , Tong-Xin Wang ₁ , Bao-Xuan Wang ₁ , Yan Shi ₁ , Bi-Xue Wang ₁ , Zheng-Rou Meng ₁ , Qing-Xi Chen ₁ , Jiang-Xing Zhuang ₂ , Yule-Yue Wang ₃

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Fish processing produces a lot of by‐products highly containing large amount of proteins which mainly consist of collagen, implying great potential value for application as nutraceutical ingredients. In present study, two kinds of sharks, Chiloscyllium plagiosum and Mustelus griseus, were used as raw material to gain three kinds of “compound peptides” (CPs) by enzymolysis, FCP (CPs from the flesh of C. plagiosum), SCP (CPs from the skin of C. plagiosum), and SMG (CPs from the skin of M. griseus). According to a series of constituent analysis, the molecule weights of FCP, SCP, and SMG were under 800 Da; amino acids composition analysis of FCP, SCP, and SMG showed that there were high glycine, proline, and hydroxyproline and low cysteine contents in SCP and SMG, which is the characteristic of collagen peptides; their total protein contents were 87.500%, 91.875%, and 95.625%, respectively; and heavy metal contents of CPs were all beneath national standards. After three kinds of CPs were administrated intragastrically to C57BL/6 mice at a total dosage of 15 g/kg, bone‐strengthening effects of SCP and SMG were manifested by osteoblasts activity promotion, bone mineral density (BMD) increase, and marrow adipocyte number decrease, yet nonsignificant effects were shown in FCP group. No index showed toxicity of SCP and SMG in subacute toxicology trial, indicating their safety as functional foods. Herein, industrial application foundation of the skins from these two sharks was explored but more efforts should subsequently be implemented for further exploitation.

中文翻译：

斜纹夜蛾和灰芥菜皮的复合肽的骨增强作用和安全性。

鱼类加工产生许多副产品，这些副产品含有大量的蛋白质，这些蛋白质主要由胶原蛋白组成，这意味着将其作为营养成分具有巨大的潜在价值。在本研究中，有两种鲨鱼，的条纹斑竹鲨和Mustelus灰色链霉菌，用作原料，通过酶解，FCP（来自的肉的CP获得3种“肽化合物”（CPS）的C.鲨），SCP（CP的C. plagiosum的皮肤）和SMG（M. griseus的皮肤的CPs）。根据一系列的成分分析，FCP，SCP和SMG的分子量小于800 Da。FCP，SCP和SMG的氨基酸组成分析表明，SCP和SMG中的甘氨酸，脯氨酸和羟脯氨酸含量高，半胱氨酸含量低，这是胶原肽的特征。总蛋白质含量分别为87.500％，91.875％和95.625％。CP的重金属含量均低于国家标准。以15 g / kg的总剂量给C57BL / 6小鼠灌胃三种CP后，成骨细胞活性增强，骨矿物质密度（BMD）增加和骨髓脂肪细胞数量显示出SCP和SMG的骨增强作用。减少，但FCP组未见明显影响。在亚急性毒理学试验中，没有指数显示出SCP和SMG的毒性，表明它们作为功能食品的安全性。在此，对这两个鲨鱼的皮的工业应用基础进行了探索，但是随后应进行更多的努力以进一步开发。

更新日期：2020-02-13

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