Volumetric muscle loss (VML) is a traumatic loss of muscle tissue that results in chronic functional impairment. When injured, skeletal muscle is capable of small‐scale repair; however, regenerative capacities are lost with VML due to a critical loss stem cells and extracellular matrix (ECM). Consequences of VML include either long‐term disability or delayed amputations of the affected limb. While the prevalence of VML is substantial, currently a successful clinical therapy has not been identified. In a previous study, an electrospun composed of polycaprolactone (PCL) and decellularized‐ECM (D‐ECM) supported satellite cell‐mediated myogenic activity in vitro. In this study, we investigate the extent to which this electrospun scaffold can support functional muscle regeneration in a murine model of VML. Experimental groups included no treatment, pure PCL treated, and PCL:D‐ECM (50:50 blend) treated VML defects. The PCL:D‐ECM scaffold treated VML muscles supported increased activity of anti‐inflammatory M2 macrophages (arginase⁺) at Day 28, compared to other experimental groups. Increased myofiber (MHC⁺) regeneration was observed histologically at both Days 7 and 28 post‐trauma in blend scaffold treated group compared to PCL treated and untreated groups. However, improvements in muscle weights and force production were not observed. Future studies would evaluate muscle function at longer time‐points post‐VML injury to allow sufficient time for reinnervation of regenerated muscle fibers.

中文翻译：

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用于体积肌肉损失的脱细胞肌肉细胞外基质的对齐纳米纤维。

容积性肌肉损失 (VML) 是肌肉组织的创伤性损失，导致慢性功能障碍。受伤时，骨骼肌能够进行小规模修复；然而，由于严重损失干细胞和细胞外基质 (ECM)，VML 会丧失再生能力。VML 的后果包括患肢的长期残疾或延迟截肢。虽然 VML 的患病率很高，但目前尚未确定成功的临床治疗方法。在之前的一项研究中，由聚己内酯 (PCL) 和脱细胞 ECM (D-ECM) 组成的静电纺丝支持体外卫星细胞介导的生肌活性。在这项研究中，我们调查了这种电纺支架在 VML 小鼠模型中支持功能性肌肉再生的程度。实验组包括不治疗、纯 PCL 处理，PCL:D-ECM（50:50 混合）处理 VML 缺陷。PCL:D-ECM 支架处理的 VML 肌肉支持抗炎 M2 巨噬细胞（精氨酸酶⁺ ) 在第 28 天，与其他实验组相比。与 PCL 治疗组和未治疗组相比，混合支架治疗组在创伤后第 7 天和第 28 天的组织学上观察到肌纤维 (MHC ⁺ ) 再生增加。然而，没有观察到肌肉重量和力量产生的改善。未来的研究将在 VML 损伤后更长的时间点评估肌肉功能，以便有足够的时间重新分配再生肌肉纤维。