Highly Efficient Biosynthesis of Hypoxanthine in Escherichia coli and Transcriptome-Based Analysis of the Purine Metabolism.,ACS Synthetic Biology

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Highly Efficient Biosynthesis of Hypoxanthine in Escherichia coli and Transcriptome-Based Analysis of the Purine Metabolism.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-02-21 , DOI: 10.1021/acssynbio.9b00396
Min Liu ₁ , Yingxin Fu ₁ , Wenjie Gao ₁ , Mo Xian ₁ , Guang Zhao ₁

Affiliation

Nucleosides and purine analogues have multiple functions in cell physiology, food additives, and pharmaceuticals, and some are produced on a large scale using different microorganisms. However, biosynthesis of purines is still lacking. In the present study, we engineered the de novo purine biosynthesis pathway, branched pathways, and a global regulator to ensure highly efficient hypoxanthine production by Escherichia coli. The engineered strain Q2973 produced 1243 mg/L hypoxanthine in fed-batch fermentation, accompanied by an extremely low accumulation of byproducts such as acetate and xanthine. We also performed global gene expression analysis to illustrate the mechanism for improving hypoxanthine production. This study demonstrated the feasibility of large-scale hypoxanthine production byan engineered E. coli strain, and provides a reference for subsequent studies on purine analogues and nucleosides.

中文翻译：

次黄嘌呤在大肠杆菌中的高效生物合成和基于转录组的嘌呤代谢分析。

核苷和嘌呤类似物在细胞生理学，食品添加剂和药物中具有多种功能，其中一些是使用不同的微生物大规模生产的。然而，仍然缺乏嘌呤的生物合成。在本研究中，我们设计了从头嘌呤的生物合成途径，分支途径和全局调节剂，以确保大肠杆菌高效生产次黄嘌呤。工程菌株Q2973在分批补料发酵中产生了1243 mg / L次黄嘌呤，同时副产物（如乙酸盐和黄嘌呤）的积累极低。我们还进行了全局基因表达分析，以说明改善次黄嘌呤生产的机制。这项研究证明了工程化大肠杆菌菌株大规模生产次黄嘌呤的可行性，

更新日期：2020-02-23

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