Abstract This work aims to understand the effect of dilute hydrogen peroxide (H2O2) solutions, used in dental whitening treatments, on the nanostructure of type I collagen fibers. Using this approach, collagen fibers were exposed to 1%, 3% and 5% H2O2 solutions, and the changes in their nanostructure were analyzed by combining topographical and phase-shift atomic force microscopy (T-AFM and PS-AFM) results with circular dichroism (CD) and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The combination of these techniques demonstrates that after exposure to 1 to 5% peroxide solutions, collagen fibers form agglomerates while the rupture of intramolecular bonds occurs, leading to collagen secondary structure loss and degradation. These results show for the first time that even dilute H2O2 solutions have a significant effect on collagen at the nanostructural level.

中文翻译：

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在牙齿美白治疗中使用稀过氧化氢后胶原纤维纳米结构的表征

摘要 这项工作旨在了解用于牙齿美白治疗的稀释过氧化氢 (H2O2) 溶液对 I 型胶原纤维纳米结构的影响。使用这种方法，将胶原纤维暴露于 1%、3% 和 5% H2O2 溶液中，并通过将形貌和相移原子力显微镜（T-AFM 和 PS-AFM）结果与圆形分析相结合来分析其纳米结构的变化。二色性 (CD) 和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 (SDS-PAGE)。这些技术的结合表明，在暴露于 1% 到 5% 的过氧化物溶液后，胶原纤维形成团块，同时发生分子内键断裂，导致胶原二级结构丢失和降解。