Next-generation physiology approaches to study microbiome function at single cell level.,Nature Reviews Microbiology

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Next-generation physiology approaches to study microbiome function at single cell level.
Nature Reviews Microbiology ( IF 69.2 ) Pub Date : 2020-02-13 , DOI: 10.1038/s41579-020-0323-1
Roland Hatzenpichler ₁ , Viola Krukenberg ₁ , Rachel L Spietz ₁ , Zackary J Jay ₁

Affiliation

The function of cells in their native habitat often cannot be reliably predicted from genomic data or from physiology studies of isolates. Traditional experimental approaches to study the function of taxonomically and metabolically diverse microbiomes are limited by their destructive nature, low spatial resolution or low throughput. Recently developed technologies can offer new insights into cellular function in natural and human-made systems and how microorganisms interact with and shape the environments that they inhabit. In this Review, we provide an overview of these next-generation physiology approaches and discuss how the non-destructive analysis of cellular phenotypes, in combination with the separation of the target cells for downstream analyses, provide powerful new, complementary ways to study microbiome function. We anticipate that the widespread application of next-generation physiology approaches will transform the field of microbial ecology and dramatically improve our understanding of how microorganisms function in their native environment.

中文翻译：

研究单细胞水平微生物组功能的下一代生理学方法。

通常无法从基因组数据或分离株的生理研究中可靠地预测细胞在其天然栖息地中的功能。研究分类和代谢多样化微生物组功能的传统实验方法受到其破坏性，低空间分辨率或低通量的限制。最近开发的技术可以为自然和人造系统中的细胞功能以及微生物如何与它们所居住的环境相互作用和形成环境提供新的见解。在这篇综述中，我们提供了这些下一代生理学方法的概述，并讨论了细胞表型的非破坏性分析，结合目标细胞的分离以进行下游分析，如何为研究微生物组功能提供强有力的，新的，互补的方法。

更新日期：2020-02-13

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