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Antioxidant and apoptotic potential of protein isolates derived from Vigna unguiculata (L.) Walp
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-02-11 , DOI: 10.1111/ijfs.14535 Dinelle Thumbrain 1 , Depika Dwarka 1 , Abe S. Gerrano 2 , John J. Mellem 1
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-02-11 , DOI: 10.1111/ijfs.14535 Dinelle Thumbrain 1 , Depika Dwarka 1 , Abe S. Gerrano 2 , John J. Mellem 1
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Projected mortalities from cancer are expected to continue escalating, with conventional chemotherapeutic regimens having extensive side effects, hence the need for therapeutics capable of terminating cancer cells selectively, through apoptosis. Therapeutic protein isolates bind specifically to target proteins inducing cell death in vitro and in vivo in various cancer cells. Therefore, this study aimed at observing the antioxidant and anticancer potential of protein isolates from five cowpea cultivars (Glenda, Embu buff, Makhatini, Veg Cowpea 2 and Veg Cowpea 3). Cytotoxicity was conducted on cancerous and non‐cancerous cell lines. Apoptosis was quantified using flow cytometry, and caspase‐3/7 activity was determined. Cytotoxicity assays revealed that Embu buff was the pre‐eminent inhibitor of cancerous cells, while also acting as a protecting mediator in a stressed non‐cancerous cell line by causing a reversal in apoptotic activity. Therefore, cowpea isolates show high potential as candidates for the therapeutic intrusion of cancer.
中文翻译：
Vi豆（Vigna unguiculata（L.）Walp）分离蛋白的抗氧化和凋亡潜力
预计来自癌症的预计死亡率将继续上升，常规化学疗法具有广泛的副作用，因此需要能够通过凋亡选择性地终止癌细胞的治疗剂。治疗性蛋白分离物与靶蛋白特异性结合，在各种癌细胞中体外和体内诱导细胞死亡。因此，本研究旨在观察五个cow豆品种（Glenda，Embu buff，Makhatini，Veg Cowpea 2和Veg Cowpea 3）分离蛋白的抗氧化和抗癌潜力。）。对癌细胞和非癌细胞系进行细胞毒性试验。使用流式细胞仪定量凋亡，并测定caspase-3 / 7活性。细胞毒性试验表明，Embu buff是癌细胞的主要抑制剂，同时还通过引起凋亡活性的逆转而在应激的非癌细胞系中充当保护介质。因此，cow豆分离株显示出作为治疗癌症的候选物的高潜力。
更新日期：2020-02-11
中文翻译：
Vi豆（Vigna unguiculata（L.）Walp）分离蛋白的抗氧化和凋亡潜力
预计来自癌症的预计死亡率将继续上升，常规化学疗法具有广泛的副作用，因此需要能够通过凋亡选择性地终止癌细胞的治疗剂。治疗性蛋白分离物与靶蛋白特异性结合，在各种癌细胞中体外和体内诱导细胞死亡。因此，本研究旨在观察五个cow豆品种（Glenda，Embu buff，Makhatini，Veg Cowpea 2和Veg Cowpea 3）分离蛋白的抗氧化和抗癌潜力。）。对癌细胞和非癌细胞系进行细胞毒性试验。使用流式细胞仪定量凋亡，并测定caspase-3 / 7活性。细胞毒性试验表明，Embu buff是癌细胞的主要抑制剂，同时还通过引起凋亡活性的逆转而在应激的非癌细胞系中充当保护介质。因此，cow豆分离株显示出作为治疗癌症的候选物的高潜力。
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