Effect of dielectric barrier discharge (DBD) plasma on the structure and antioxidant activity of bovine serum albumin (BSA),International Journal of Food Science & Technology

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Effect of dielectric barrier discharge (DBD) plasma on the structure and antioxidant activity of bovine serum albumin (BSA)
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-02-11 , DOI: 10.1111/ijfs.14536
Zhi‐Wei Liu ₁ , Muhammad Faisal Manzoor ₂ , Yi‐Cheng Tan ₁ , Muhammad Inam‐ur‐Raheem ₃ , Rana Muhammad Aadil ₃

Affiliation

This study was aimed to determine the influence of dielectric barrier discharge (DBD) plasma with a plasma intensity (20 and 40 kV) and treatment times (0, 2, 4, 6 and 8 min) on the physicochemical, structural and antioxidant properties of bovine serum albumin (BSA) solution. In results, the free sulphydryl content, pH and free amino group contents, while carbonyl groups and surface hydrophobicity were increased after the plasma treatment. After plasma treatment, there is also an increase in the DPPH and FRAP assay that was associated with the changes in the structure of the peptides chain. The complementary techniques were employed to identify the protein structural modifications by plasma treatment. These changes were more severe at the most extended treatment duration and plasma intensity. The high energetic and oxidising species carried by plasma may have significant associations on the structural and physical properties of BSA.

中文翻译：

介质阻挡放电（DBD）血浆对牛血清白蛋白（BSA）的结构和抗氧化活性的影响

这项研究的目的是确定等离子强度（20和40 kV）和处理时间（0、2、4、6和8分钟）的电介质阻挡放电（DBD）等离子体对其物理化学，结构和抗氧化性能的影响牛血清白蛋白（BSA）溶液。结果，等离子体处理后，游离巯基含量，pH和游离氨基含量增加，而羰基和表面疏水性增加。血浆处理后，DPPH和FRAP分析也有所增加，这与肽链结构的变化有关。采用互补技术通过血浆处理鉴定蛋白质的结构修饰。在最延长的治疗时间和血浆强度下，这些变化更为严重。

更新日期：2020-02-11

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