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Detergent‐Assisted Braking of Peptide Translocation through a Single‐Layer Molybdenum Disulfide Nanopore
Small Methods ( IF 10.7 ) Pub Date : 2020-02-12 , DOI: 10.1002/smtd.201900822 Wei Si 1 , Qianyi Sun 1 , Chang Chen 1 , Meng Yu 1 , Jingjie Sha 1 , Yin Zhang 1 , Yajing Kan 1 , Yunfei Chen 1
Small Methods ( IF 10.7 ) Pub Date : 2020-02-12 , DOI: 10.1002/smtd.201900822 Wei Si 1 , Qianyi Sun 1 , Chang Chen 1 , Meng Yu 1 , Jingjie Sha 1 , Yin Zhang 1 , Yajing Kan 1 , Yunfei Chen 1
Affiliation
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Protein sequencing holds the key to unveiling protein dynamics, which is significant for molecular diagnostics and drug design. Single‐molecule sensing based on 2D nanopores is a versatile technique for realizing low‐cost and high‐throughput protein sequencing targets. However, the remaining challenge is that protein translocates rapidly through nanopores, where few current signals can be gathered for accurate protein sequencing. It is theoretically found that adding nonionic detergents to a solution can slow protein molecules by almost one order when they permeate through a 1.4 nm MoS2 nanopore that is slightly larger than the diameter of the peptide but smaller than the diameter of the protein–detergent complex. This slowdown is due to the strong interaction between the hydrophobic parts of the peptide and detergents. Although detergent molecules are added to the system, ionic current blockade levels can be observed for different residues, indicating that the nonionic detergents are excellent candidates for controlling protein translocation process but do not affect the discrimination of single amino acids. This theoretical work motivates experimental efforts in this area and paves a new way for protein manipulation that brings the nanopore technique closer to satisfying the requirement of high‐precision nanopore protein sequencing.
中文翻译：
洗涤剂辅助的通过单层二硫化钼纳米孔进行的肽移位制动
蛋白质测序是揭示蛋白质动力学的关键，这对分子诊断和药物设计具有重要意义。基于二维纳米孔的单分子传感是一种用于实现低成本和高通量蛋白质测序目标的通用技术。然而，剩下的挑战是蛋白质通过纳米孔快速转运，在纳米孔中几乎没有电流信号可以收集以进行准确的蛋白质测序。从理论上讲，向溶液中添加非离子型去污剂可以使蛋白质分子透过1.4 nm MoS 2的速度降低近一个数量级。纳米孔，其直径略大于肽的直径，但小于蛋白洗涤剂复合物的直径。这种减慢是由于肽的疏水部分和去污剂之间的强相互作用。尽管将去污剂分子添加到系统中，但可以观察到不同残基的离子电流阻滞水平，这表明非离子去污剂是控制蛋白质转运过程的极佳候选者，但不影响单个氨基酸的区分。这项理论工作激发了这一领域的实验工作，并为蛋白质操纵开辟了一条新途径，使纳米孔技术更接近于满足高精度纳米孔蛋白质测序的要求。
更新日期：2020-02-12
中文翻译：
洗涤剂辅助的通过单层二硫化钼纳米孔进行的肽移位制动
蛋白质测序是揭示蛋白质动力学的关键，这对分子诊断和药物设计具有重要意义。基于二维纳米孔的单分子传感是一种用于实现低成本和高通量蛋白质测序目标的通用技术。然而，剩下的挑战是蛋白质通过纳米孔快速转运，在纳米孔中几乎没有电流信号可以收集以进行准确的蛋白质测序。从理论上讲，向溶液中添加非离子型去污剂可以使蛋白质分子透过1.4 nm MoS 2的速度降低近一个数量级。纳米孔，其直径略大于肽的直径，但小于蛋白洗涤剂复合物的直径。这种减慢是由于肽的疏水部分和去污剂之间的强相互作用。尽管将去污剂分子添加到系统中，但可以观察到不同残基的离子电流阻滞水平，这表明非离子去污剂是控制蛋白质转运过程的极佳候选者，但不影响单个氨基酸的区分。这项理论工作激发了这一领域的实验工作，并为蛋白质操纵开辟了一条新途径，使纳米孔技术更接近于满足高精度纳米孔蛋白质测序的要求。
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