BACKGROUND The effect of ginsenosides on the growth and apoptosis of human lens epithelial (HLE) B3 cells exposed to H2O2 was investigated. In addition, the effect of ginsenosides on gene expression in HLE-B3 cells was analyzed using microarray assays to determine its molecular mechanism. METHODS HLE-B3 cells were treated with 1.75 M H2O2 in the presence or absence of 5, 10 or 20 μM ginsenosides. Cell viability and apoptosis were examined by MTT assays and flow cytometry, respectively, at 24 to 120 h after the treatment. Furthermore, HLE-B3 cells were treated with 20 μM ginsenosides for 8 days and total RNA was isolated and analyzed using the Affymetrix GeneChip Array. Principal component analysis was performed to visualize the microarray data. RESULTS Addition of ginsenosides significantly alleviated the growth inhibitory effect of H2O2 on HLE-B3 cells and the percentage of viable cells was increased by more than 3 folds. Flow cytometric analysis showed that 6.16 ± 0.29% of H2O2-treated HLE-B3 cells were early apoptotic cells, and the percentage was reduced to 4.78 ± 0.16% (P < 0.05) in the presence of 20 μM ginsenosides. Principal component analysis revealed that ginsenoside caused extensive changes in gene expression in HLE-B3 cells. A total of 6219 genes showed significant differential expression in HLE-B3 cells treated with ginsenoside; among them, 2552 (41.0%) genes were significantly upregulated, whereas 3667 (59.0%) genes were significantly downregulated. FOXN2, APP and RAD23B were the top three upregulated genes while WSB1, PSME4 and DCAF7 were the top three downregulated genes in HLE-B3 cells treated with ginsenosides. CONCLUSION Ginsenosides induce extensive changes in the expression of genes involved in multiple signaling pathways, including apoptotic signaling pathway and DNA damage response signaling pathway. Ginsenosides alleviate H2O2-induced suppression of the growth of HLB cells and inhibit H2O2-induced apoptosis of HLB cells.

中文翻译：

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人参皂甙可诱导人晶状体上皮细胞基因表达的广泛变化并抑制氧化应激诱导的细胞凋亡。

背景技术研究了人参皂甙对暴露于H2O2的人晶状体上皮（HLE）B3细胞生长和凋亡的影响。此外，使用微阵列分析法分析了人参皂甙对HLE-B3细胞基因表达的影响，以确定其分子机制。方法在存在或不存在5、10或20μM人参皂甙的情况下，用1.75 M H2O2处理HLE-B3细胞。在处理后24至120小时，分别通过MTT测定和流式细胞术检查细胞活力和凋亡。此外，将HLE-B3细胞用20μM人参皂甙处理8天，并使用Affymetrix GeneChip Array分离并分析总RNA。进行主成分分析以可视化微阵列数据。结果人参皂苷的添加明显减轻了H2O2对HLE-B3细胞的生长抑制作用，并且存活细胞的百分比增加了3倍以上。流式细胞仪分析显示，经H2O2处理的HLE-B3细胞中有6.16±0.29％是早期凋亡细胞，在存在20μM人参皂甙的情况下，该百分比降低至4.78±0.16％（P <0.05）。主成分分析表明，人参皂苷引起了HLE-B3细胞基因表达的广泛变化。人参皂甙处理的HLE-B3细胞中共有6219个基因显示出明显的差异表达；其中，显着上调了2552个基因（41.0％），而显着下调了3667个（59.0％）基因。FOXN2，APP和RAD23B是前三个上调的基因，而WSB1，PSME4和DCAF7是用人参皂甙处理的HLE-B3细胞中排名前三的下调基因。结论人参皂苷可诱导涉及多种信号通路的基因表达发生广泛变化，这些信号通路包括凋亡信号通路和DNA损伤反应信号通路。人参皂苷减轻H2O2诱导的HLB细胞生长抑制，并抑制H2O2诱导的HLB细胞凋亡。