The US Environmental protection agency (EPA) has published guidance that includes test procedures for evaluating indoor exposure to chemicals from products. One of the test procedures represents the migration test for evaluating potential dermal exposure from home furniture. Such an evaluation involves the chemical measurement of the sweat which is currently unavailable in the literature. The objective of this project was to develop and validate an analytical method for quantification of migration of 4,4'-methylenediphenyl diisocyanate (MDI), 2,6-toluene diisocyanate (2,6-TDI) and 2,4-toluene diisocyanate (2,4-TDI) from a polyurethane (PU) flexible foam to artificial sweat that meets the recommendations of the EPA test protocol. Following the EPA protocol, six synthetic sweat solutions were prepared and used in evaluation of isocyanate recovery performance. The migration tests were conducted using five foam types that were chosen and supplied by PU foam manufacturers to represent the types most commonly found in commercial products, and with formulations anticipated to have the highest potential residual TDI or MDI. Migration tests were conducted using glass fiber filters (GFF) coated with 1-(2-methoxyphenyl)piperazine (1,2-MP) and analyzed using HPLC equipped with a UV detector for quantification and a MS detector to qualify peaks. The detection limits of the method were 0.002 µg/mL for 2,6-TDI, 0.011 µg/mL for 2,4-TDI, and 0.003 µg/mL for MDI. Quantification limits were 0.006 µg/mL, 0.037 µg/mL, and 0.010 µg/mL, respectively. The recovery tests on a Teflon surface for 5 of the 6 EPA-recommended synthetic sweat solutions indicate the recovery percentage was approximately 80% for diisocyanates. Recovery for the sixth sweat solution was low, approximately 30%. TDI and MDI migration was not observed when testing was conducted on foam samples.

中文翻译：

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开发了一种通过HPLC-UV-MS定量分析甲苯二异氰酸酯和亚甲基二苯基二异氰酸酯从聚氨酯泡沫样品表面迁移至人造汗液的方法。

美国环境保护署（EPA）已发布指南，其中包括用于评估室内暴露于产品中化学物质的测试程序。测试程序之一是用于评估家用家具潜在的皮肤暴露的迁移测试。这样的评估涉及汗液的化学测量，这在文献中是目前尚不可用的。该项目的目的是开发和验证一种定量分析4,4'-亚甲基二苯基二异氰酸酯（MDI），2,6-甲苯二异氰酸酯（2,6-TDI）和2,4-甲苯二异氰酸酯（从聚氨酯（PU）柔性泡沫到人工汗液的2,4-TDI）均符合EPA测试协议的建议。按照EPA协议，制备了六种合成汗液并将其用于评估异氰酸酯的回收性能。使用PU泡沫制造商选择和提供的五种泡沫类型进行迁移测试，以代表在商业产品中最常见的类型，并预期配方具有最高的潜在残留TDI或MDI。使用涂有1-（2-甲氧基苯基）哌嗪（1,2-MP）的玻璃纤维滤膜（GFF）进行迁移测试，并使用配备有UV检测器进行定量分析和MS检测器鉴定峰的HPLC分析。该方法的检出限分别为：2,6-TDI为0.002 µg / mL，2,4-TDI为0.011 µg / mL，MDI为0.003 µg / mL。定量限分别为0.006 µg / mL，0.037 µg / mL和0.010 µg / mL。在6种EPA推荐的合成汗液中，有5种在特氟龙表面的回收率测试表明，二异氰酸酯的回收率约为80％。第六种汗液的回收率很低，约为30％。对泡沫样品进行测试时，未观察到TDI和MDI迁移。