Label-free stimulated Raman scattering imaging reveals silicone breast implant material in tissue.,Journal of Biophotonics

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Label-free stimulated Raman scattering imaging reveals silicone breast implant material in tissue.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-02-23 , DOI: 10.1002/jbio.201960197
Ludo van Haasterecht _{1,

2} , Liron Zada _{1,

3} , Robert W Schmidt ₁ , Erik de Bakker _{2,

4} , Ellis Barbé ₅ , Heather A Leslie ₃ , A Dick Vethaak _{3,

6} , Susan Gibbs _{4,

7} , Johannes F de Boer ₁ , Frank B Niessen ₂ , Paul P M van Zuijlen _{2,

8} , Marie Louise Groot ₁ , Freek Ariese ₁

Affiliation

Millions of women worldwide have silicone breast implants. It has been reported that implant failure occurs in approximately a tenth of patients within 10 years, and the consequences of dissemination of silicone debris are poorly understood. Currently, silicone detection in histopathological slides is based on morphological features as no specific immunohistochemical technique is available. Here, we show the feasibility and sensitivity of stimulated Raman scattering (SRS) imaging to specifically detect silicone material in stained histopathological slides, without additional sample treatment. Histology slides of four periprosthetic capsules from different implant types were obtained after explantation, as well as an enlarged axillary lymph node from a patient with a ruptured implant. SRS images coregistered with bright‐field images revealed the distribution and quantity of silicone material in the tissue. Fast and high‐resolution imaging of histology slides with molecular specificity using SRS provides an opportunity to investigate the role of silicone debris in the pathophysiology of implant‐linked diseases.

中文翻译：

无标记刺激的拉曼散射成像揭示了组织中的有机硅乳房植入物材料。

全球数以百万计的妇女拥有硅胶乳房植入物。据报道，植入失败在10年内约有十分之一的患者发生，而硅树脂碎片散布的后果知之甚少。目前，组织病理学玻片中的有机硅检测是基于形态学特征的，因为尚无特定的免疫组织化学技术。在这里，我们展示了刺激拉曼散射（SRS）成像技术在不进行额外样品处理的情况下特异性检测染色的病理组织切片中的硅树脂材料的可行性和敏感性。植入后，获得了来自不同植入物类型的四个假体周围囊的组织学切片，以及植入物破裂的患者的腋窝淋巴结肿大。SRS图像与明场图像共同记录，揭示了组织中硅树脂材料的分布和数量。使用SRS对分子组织特异性的组织切片进行快速，高分辨率的成像，为研究硅屑在植入物相关疾病的病理生理学中的作用提供了机会。

更新日期：2020-02-23

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