Decellularization involves removal of cellular material from tissue which results in a scaffold material consisting of only the extra cellular matrix (ECM). The effect of each individual decellularizing detergent on the final ECM scaffold and how that may differ from the combined use of these detergents is currently a gap in decellularization methodologies. This study evaluates the hypothesis that a synergistic effect exists when commonly used decellularization detergents are combined. This was evaluated with regard to decellularization efficiency, tissue strength, and collagen structure. Bovine pericardium was decellularized using a combination of 0.5% sodium dodecyl sulfate (SDS), 1% sodium deoxycholate (SDC) and 1% TritonX‐100, and compared to the use of each detergent individually. The combined detergent decellularization protocol showed effective decellularization (p = .004), with minimal effects on tissue strength (p = .21) and structure (p = .21). Use of detergents individually, resulted in detrimental effects on tissue structure and integrity or ineffective decellularization. This study shows a synergistic relationship between SDS, SDC and TritonX‐100 when combined at specific concentrations. The use of detergents in combination instead of individually appears to be superior, as it results in less ECM damage and improved decellularization effectivity.

中文翻译：

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洗涤剂组合中的协同作用产生了卓越的脱细胞牛心包细胞外基质支架。

去细胞化包括从组织中去除细胞材料，这会产生仅由细胞外基质 (ECM) 组成的支架材料。每个单独的脱细胞洗涤剂对最终 ECM 支架的影响以及这与这些洗涤剂的组合使用有何不同，目前是脱细胞方法的一个空白。本研究评估了当常用的去细胞洗涤剂组合时存在协同效应的假设。这在脱细胞效率、组织强度和胶原结构方面进行了评估。使用 0.5% 十二烷基硫酸钠 (SDS)、1% 脱氧胆酸钠 (SDC) 和 1% TritonX-100 的组合对牛心包进行脱细胞，并与单独使用每种洗涤剂进行比较。p = .004)，对组织强度 ( p = .21) 和结构 ( p = .21) 的影响最小。单独使用洗涤剂会导致对组织结构和完整性的不利影响或无效的脱细胞。该研究表明，当以特定浓度组合时，SDS、SDC 和 TritonX-100 之间存在协同关系。组合使用洗涤剂而不是单独使用似乎更胜一筹，因为它可以减少 ECM 损伤并提高脱细胞效果。