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Wide-field, high-resolution lensless on-chip microscopy via near-field blind ptychographic modulation.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-02-19 , DOI: 10.1039/c9lc01027k
Shaowei Jiang 1 , Jiakai Zhu 1 , Pengming Song 2 , Chengfei Guo 1 , Zichao Bian 1 , Ruihai Wang 1 , Yikun Huang 1 , Shiyao Wang 3 , He Zhang 1 , Guoan Zheng 4
Affiliation  

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning the unknown diffuser to different x-y positions, we acquire a sequence of modulated intensity images for quantitative object recovery. Different from previous ptychographic implementations, we employ a unit magnification configuration with a Fresnel number of ∼50 000, which is orders of magnitude higher than those of previous ptychographic setups. The unit magnification configuration allows us to have the entire sensor area, 6.4 mm by 4.6 mm, as the imaging field of view. The ultra-high Fresnel number enables us to directly recover the positional shift of the diffuser in the phase retrieval process, addressing the positioning accuracy issue plaguing regular ptychographic experiments. In our implementation, we use a low-cost, DIY scanning stage to perform blind diffuser modulation. Precise mechanical scanning that is critical in conventional ptychography experiments is no longer needed in our setup. We further employ an up-sampling phase retrieval scheme to bypass the resolution limit set by the imager pixel size and demonstrate a half-pitch resolution of 0.78 μm. We validate the imaging performance via in vitro cell cultures, transparent and stained tissue sections, and a thick biological sample. We show that the recovered quantitative phase map can be used to perform effective cell segmentation of a dense yeast culture. We also demonstrate 3D digital refocusing of the thick biological sample based on the recovered wavefront. The reported platform provides a cost-effective and turnkey solution for large field-of-view, high-resolution, and quantitative on-chip microscopy. It is adaptable for a wide range of point-of-care-, global-health-, and telemedicine-related applications.

中文翻译:

通过近场盲态码型调制实现的宽视野,高分辨率,无透镜片上显微镜。

我们报告了一种新型的无透镜片上显微镜平台,该平台基于近场盲态声像图调制。在这个平台上,我们在物体和图像传感器之间放置了一个薄的扩散器,用于光波调制。通过盲目地将未知的扩散器扫描到不同的xy位置,我们获得了一系列调制强度图像,用于定量的对象恢复。与以前的谱图实现不同,我们采用单位放大倍数配置,菲涅耳数约为50,000,这比以前的谱图设置要高几个数量级。单位放大倍数配置使我们能够将整个传感器区域(6.4毫米乘4.6毫米)作为成像视场。极高的菲涅耳数使我们能够直接恢复相位恢复过程中扩散器的位置偏移,解决了困扰常规谱图实验的定位精度问题。在我们的实现中,我们使用低成本的DIY扫描台来执行盲扩散器调制。在我们的设置中,不再需要在传统笔迹学实验中至关重要的精确机械扫描。我们进一步采用了上采样相位检索方案来绕过由成像器像素尺寸设置的分辨率极限,并演示了0.78μm的半间距分辨率。我们通过体外细胞培养,透明和染色的组织切片以及厚厚的生物样品来验证成像性能。我们表明,回收的定量相图可用于执行密集的酵母培养物的有效细胞分割。我们还演示了基于恢复的波前的厚生物样品的3D数字重新聚焦。报告的平台为大型视野,高分辨率和定量芯片上显微技术提供了一种经济高效的统包解决方案。它适用于广泛的即时护理,全球健康和远程医疗相关应用。
更新日期:2020-03-19
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