当前位置：
X-MOL 学术
›
J. Proteomics
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Proteomic analysis revealed different responses to hypergravity of soleus and extensor digitorum longus muscles in mice.
Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-02-12 , DOI: 10.1016/j.jprot.2020.103686 Takashi Ohira 1 , Yoko Ino 1 , Yusuke Nakai 1 , Hironobu Morita 2 , Ayuko Kimura 1 , Yoichi Kurata 1 , Hiroyuki Kagawa 1 , Mitsuo Kimura 1 , Kenji Egashira 1 , Shunsuke Moriya 3 , Kyoko Hiramatsu 3 , Masao Kawakita 3 , Yayoi Kimura 1 , Hisashi Hirano 1
Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-02-12 , DOI: 10.1016/j.jprot.2020.103686 Takashi Ohira 1 , Yoko Ino 1 , Yusuke Nakai 1 , Hironobu Morita 2 , Ayuko Kimura 1 , Yoichi Kurata 1 , Hiroyuki Kagawa 1 , Mitsuo Kimura 1 , Kenji Egashira 1 , Shunsuke Moriya 3 , Kyoko Hiramatsu 3 , Masao Kawakita 3 , Yayoi Kimura 1 , Hisashi Hirano 1
Affiliation
|
Investigating protein abundance profiles is important to understand the differences in the slow and fast skeletal muscle characteristics. The profiles in soleus (Sol) and extensor digitorum longus (EDL) muscles in mice exposed to 1 g or 3 g for 28 d were compared. The biological implications of the profiles revealed that hypergravity exposure activated a larger number of pathways involved in protein synthesis in Sol. In contrast, the inactivation of signalling pathways involved in oxidative phosphorylation were conspicuous in EDL. These results suggested that the reactivity of molecular pathways in Sol and EDL differed. Additionally, the levels of spermidine synthase and spermidine, an important polyamine for cell growth, increased in both muscles following hypergravity exposure, whereas the level of spermine oxidase (SMOX) increased in EDL alone. The SMOX level was negatively correlated with spermine content, which is involved in muscle atrophy, and was higher in EDL than Sol, even in the 1 g group. These results indicated that the contribution of SMOX to the regulation of spermidine and spermine contents in Sol and EDL differed. However, contrary to expectations, the difference in the SMOX level did not have a significant impact on the growth of these muscles following hypergravity exposure. SIGNIFICANCE: The skeletal muscle-specific protein abundance profiles result in differences in the characteristics of slow and fast skeletal muscles. We investigated differences in the profiles in mouse slow-twitch Sol and fast-twitch EDL muscles following 28-d of 1 g and 3 g exposure by LC-MS/MS analysis and label-free quantitation. A two-step solubilisation of the skeletal muscle proteins increased the coverage of proteins identified by LC-MS/MS analysis. Additionally, this method reduced the complexity of samples more easily than protein or peptide fractionation by SDS-PAGE and offline HPLC while maintaining the high operability of samples and was reproducible. A larger number of hypergravity-responsive proteins as well as a prominent increase in the wet weights was observed in Sol than EDL muscles. The biological implications of the difference in the protein abundance profiles in 1 g and 3 g groups revealed that the reactivity of each molecular pathway in Sol and EDL muscles to hypergravity exposure differed significantly. In addition, we found that the biosynthetic and interconversion pathway of polyamines, essential factors for cell growth and survival in mammals, was responsive to hypergravity exposure; spermidine and spermine contents in Sol and EDL muscles were regulated by different mechanisms even in the 1 g group. However, our results indicated that the difference in the mechanism regulating polyamine contents is unlikely to have a significant effect on the differences in Sol and EDL muscle growth following hypergravity exposure.
中文翻译：
蛋白质组学分析显示，小鼠比目鱼肌和趾长伸肌的超重反应不同。
研究蛋白质丰度概况对于了解慢速和快速骨骼肌特征的差异很重要。比较暴露于1 g或3 g达28 d的小鼠的比目鱼（Sol）和趾长伸肌（EDL）肌肉的分布。这些图谱的生物学意义表明，超重力暴露激活了参与Sol中蛋白质合成的大量途径。相反，在EDL中，涉及氧化磷酸化的信号通路的失活是明显的。这些结果表明，Sol和EDL中分子途径的反应性不同。另外，超重力暴露后，两块肌肉中亚精胺合酶和亚精胺（一种重要的细胞生长多胺）的水平均增加，而仅EDL中精胺氧化酶（SMOX）的水平升高。SMOX水平与精子含量负相关，精子含量与肌肉萎缩有关，即使在1 g组中，EDL中的精胺含量也高于Sol。这些结果表明，SMOX对Sol和EDL中亚精胺和亚精胺含量的调节作用不同。但是，与预期相反，超重力暴露后SMOX水平的差异对这些肌肉的生长没有显着影响。意义：骨骼肌特异的蛋白质丰度概况导致慢速和快速骨骼肌特征的差异。我们通过LC-MS / MS分析和无标记定量研究了28 d 1 g和3 g暴露后小鼠慢肌Sol和快肌EDL肌肉的轮廓差异。骨骼肌蛋白质的两步溶解可增加通过LC-MS / MS分析鉴定的蛋白质的覆盖率。此外，该方法比通过SDS-PAGE和离线HPLC进行蛋白质或肽分级分离更容易地降低了样品的复杂性，同时又保持了样品的高可操作性并且具有重现性。与EDL肌肉相比，在Sol中观察到大量的超重力反应蛋白以及湿重的显着增加。1 g和3 g组蛋白质丰度分布差异的生物学意义表明，Sol和EDL肌肉中每个分子途径对超重力暴露的反应性显着不同。此外，我们发现多胺的生物合成和相互转化途径是哺乳动物细胞生长和存活的重要因素，对超重力暴露有反应；即使在1 g组中，Sol和EDL肌肉中亚精胺和亚精胺的含量也受不同机制的调节。但是，我们的结果表明，调节多胺含量的机制差异不太可能对超重力暴露后的Sol和EDL肌肉生长差异产生重大影响。
更新日期：2020-02-12
中文翻译：
蛋白质组学分析显示，小鼠比目鱼肌和趾长伸肌的超重反应不同。
研究蛋白质丰度概况对于了解慢速和快速骨骼肌特征的差异很重要。比较暴露于1 g或3 g达28 d的小鼠的比目鱼（Sol）和趾长伸肌（EDL）肌肉的分布。这些图谱的生物学意义表明，超重力暴露激活了参与Sol中蛋白质合成的大量途径。相反，在EDL中，涉及氧化磷酸化的信号通路的失活是明显的。这些结果表明，Sol和EDL中分子途径的反应性不同。另外，超重力暴露后，两块肌肉中亚精胺合酶和亚精胺（一种重要的细胞生长多胺）的水平均增加，而仅EDL中精胺氧化酶（SMOX）的水平升高。SMOX水平与精子含量负相关，精子含量与肌肉萎缩有关，即使在1 g组中，EDL中的精胺含量也高于Sol。这些结果表明，SMOX对Sol和EDL中亚精胺和亚精胺含量的调节作用不同。但是，与预期相反，超重力暴露后SMOX水平的差异对这些肌肉的生长没有显着影响。意义：骨骼肌特异的蛋白质丰度概况导致慢速和快速骨骼肌特征的差异。我们通过LC-MS / MS分析和无标记定量研究了28 d 1 g和3 g暴露后小鼠慢肌Sol和快肌EDL肌肉的轮廓差异。骨骼肌蛋白质的两步溶解可增加通过LC-MS / MS分析鉴定的蛋白质的覆盖率。此外，该方法比通过SDS-PAGE和离线HPLC进行蛋白质或肽分级分离更容易地降低了样品的复杂性，同时又保持了样品的高可操作性并且具有重现性。与EDL肌肉相比，在Sol中观察到大量的超重力反应蛋白以及湿重的显着增加。1 g和3 g组蛋白质丰度分布差异的生物学意义表明，Sol和EDL肌肉中每个分子途径对超重力暴露的反应性显着不同。此外，我们发现多胺的生物合成和相互转化途径是哺乳动物细胞生长和存活的重要因素，对超重力暴露有反应；即使在1 g组中，Sol和EDL肌肉中亚精胺和亚精胺的含量也受不同机制的调节。但是，我们的结果表明，调节多胺含量的机制差异不太可能对超重力暴露后的Sol和EDL肌肉生长差异产生重大影响。
京公网安备 11010802027423号