In this study, we investigated how Oroxylum indicum leaf and fruit extracts affect the viability and migration of MCF-7 breast cancer cells and the mechanisms of action responsible for these effects. MCF-7 cells treated with the extracts were examined using the sulforhodamine B, colony formation and caspase 3 activity assays, and by Western blotting. O. indicum extracts were found to inhibit MCF-7 cell growth in a concentration- and time-dependent manner, with 48 h IC₅₀ values of 57.02 ± 2.85 μg/mL and 131.3 ± 19.2 μg/mL for leaf and fruit extracts, respectively. Further, the O. indicum leaf extract caused a reduction in MCF-7 cell viability, induction of MCF-7 cell apoptosis and ROS formation, and an increase in caspase 3 activity. Also, the two extracts inhibited MCF-7 cell migration and reduced both MMP 9 and ICAMP1 gene expression and MMP9 protein expression. Additionally, O. indicum extracts greatly reduced expression of the cell cycle regulatory protein Rac1 in the mevalonate pathway. In summary, O. indicum leaf and fruit extracts reduce breast cancer cell growth, cell viability and cell migration. O. indicum constituents could, therefore, be useful for augmenting the activity of chemotherapeutic drugs employed to treat breast cancer.

在这项研究中，我们调查了印度香叶和果实的提取物如何影响MCF-7乳腺癌细胞的活力和迁移以及造成这些影响的作用机制。使用磺基罗丹明B，集落形成和caspase 3活性测定以及Western印迹检查了用提取物处理过的MCF-7细胞。发现印度梧桐提取物以浓度和时间依赖性的方式抑制MCF-7细胞的生长，叶子和果实提取物的48 h IC ₅₀值分别为57.02±2.85μg/ mL和131.3±19.2μg/ mL。 。此外，印度洋叶提取物导致MCF-7细胞活力降低，MCF-7细胞凋亡和ROS形成诱导以及caspase 3活性增加。同样，两种提取物抑制MCF-7细胞迁移，并降低MMP 9和ICAMP1基因表达以及MMP9蛋白表达。此外，印度梧桐提取物大大降低了甲羟戊酸途径中细胞周期调节蛋白Rac1的表达。总之，印度梧桐叶和果实提取物可降低乳腺癌细胞的生长，细胞活力和细胞迁移。因此，O.indicum成分可用于增强用于治疗乳腺癌的化学治疗药物的活性。