Abstract Dysosma versipellis (Hance) M. Chang is an endemic and endangered Chinese medicinal herb. Podophyllotoxin, a lead compound for the semi-synthesis of clinically useful anticancer compounds, is the main active aryltetralin lignan accumulated in D. versipellis. In this study, morphology and physiology traits in response to podophyllotoxin accumulation and its biosynthetic pathway genes expression were investigated in D. versipellis plants grown at low temperature (4∼6 °C) and greenhouse (25∼30 °C) temperature. Our study found that low temperature significantly increased plant biomass (2.7-fold), chlorophyll, and carotenoid content compared to greenhouse temperature. The mRNA expression analysis of podophyllotoxin pathway genes secoisolariciresinol dehydrogenase and pinoresionol lariciresinol reductase expression levels were also up-regulated by low temperature. In addition, plants grown at low temperature markedly increased podophyllotoxin accumulation to 3.49-fold in rhizome of D. versipellis than the greenhouse temperature. These results suggest that plants at low temperature enhance the accumulation of podophyllotoxin in rhizome of D. versipellis by over-expressing podophyllotoxin pathway genes. This study finding could be useful in the producing pharmaceutically important podophyllotoxin without overexploiting this endangered species.

中文翻译：

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低温增强了鬼臼毒素的积累，相对于其生物合成途径基因在 Dysosma versipellis (Hance) M. Cheng – 药学上重要的药用植物中的表达

摘要 丹参是一种地方性濒危中药材。鬼臼毒素是半合成临床上有用的抗癌化合物的先导化合物，是在百合花中积累的主要活性芳四林木脂素。在这项研究中，研究了在低温（4~6°C）和温室（25~30°C）温度下生长的花椒植物响应鬼臼毒素积累的形态学和生理学特征及其生物合成途径基因表达。我们的研究发现，与温室温度相比，低温显着增加了植物生物量（2.7 倍）、叶绿素和类胡萝卜素含量。鬼臼毒素通路基因开环异落叶松树脂醇脱氢酶和松果松醇落叶松树脂醇还原酶的 mRNA 表达水平也因低温而上调。此外，与温室温度相比，在低温下生长的植物在花椒根茎中的鬼臼毒素积累显着增加了 3.49 倍。这些结果表明，低温下的植物通过过量表达鬼臼毒素途径基因来增强花椒根茎中鬼臼毒素的积累。该研究发现可用于生产具有药用价值的鬼臼毒素，而不会过度开发这种濒危物种。番红花比温室温度。这些结果表明，低温下的植物通过过量表达鬼臼毒素途径基因来增强花椒根茎中鬼臼毒素的积累。该研究发现可用于生产具有药用价值的鬼臼毒素，而不会过度开发这种濒危物种。番红花比温室温度。这些结果表明，低温下的植物通过过量表达鬼臼毒素途径基因来增强花椒根茎中鬼臼毒素的积累。该研究发现可用于生产具有药用价值的鬼臼毒素，而不会过度开发这种濒危物种。