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Auxin and Cytokinin Synergism Augmenting Biomass and Lipid Production in Microalgae Desmodesmus sp. JS07
Process Biochemistry ( IF 3.7 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.procbio.2020.02.012
Jyoti Singh , Deeksha Jain , Pragati Agarwal , R.P. Singh

Abstract Microalgae are considered as a promising resource for biodiesel. Nevertheless, their commercial exploitation necessitates considerable impetus towards the development of approaches for increased biomass and lipid production. The present work elucidates the impact of exogenously supplemented auxins, i.e., indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and indole-3-propionic acid (IPA) and cytokinins, i.e., benzylaminopurine (BAP) and thidiazuron (TDZ) on biomass, lipid content and fatty acid profile of Desmodesmus sp. JS07. Among auxins, IBA improved the biomass and lipid content up to 1.96 ± 0.11 g/L and 34.88 ± 3.87 %, respectively while BAP among cytokinins increased the biomass and lipid content up to 1.88 ± 0.061 g/L and 31.84 ± 1.33 % respectively. Further, the cumulative impact of IBA (10 mg/L) and BAP (5 mg/L) resulted in their synergistic effect by stimulating biomass and lipid content up to 2.34 ± 0.032 g/L and 42.43 ± 1.88 % respectively. Auxins stimulated the superoxide dismutase activity, and cytokinins increased the enzymes (catalase and ascorbate peroxidase), scavenging reactive oxidative species, thereby regulating ROS homeostasis in microalgae. A significant alteration in the fatty acid profile owing to the type and dosage of phytohormones was detected. Hence, the strategy employing phytohormones could prove to be a meaningful approach for biofuel production.

中文翻译:

生长素和细胞分裂素协同作用增强微藻 Desmodesmus sp. 的生物量和脂质生产。JS07

摘要 微藻被认为是一种很有前景的生物柴油资源。然而,它们的商业开发需要相当大的推动力来开发增加生物量和脂质生产的方法。目前的工作阐明了外源性补充的生长素的影响,即吲哚-3-乙酸 (IAA)、吲哚-3-丁酸 (IBA) 和吲哚-3-丙酸 (IPA) 和细胞分裂素,即苄氨基嘌呤 (BAP) ) 和噻二唑脲 (TDZ) 对 Desmodesmus sp. 的生物量、脂质含量和脂肪酸谱的影响。JS07。在生长素中,IBA 将生物量和脂质含量分别提高至 1.96 ± 0.11 g/L 和 34.88 ± 3.87 %,而细胞分裂素中的 BAP 将生物量和脂质含量分别提高至 1.88 ± 0.061 g/L 和 31.84 ± 1.33 %。更多,IBA (10 mg/L) 和 BAP (5 mg/L) 的累积影响通过刺激生物量和脂质含量分别高达 2.34 ± 0.032 g/L 和 42.43 ± 1.88 % 产生协同效应。生长素刺激超氧化物歧化酶活性,细胞分裂素增加酶(过氧化氢酶和抗坏血酸过氧化物酶),清除活性氧化物质,从而调节微藻中的 ROS 稳态。由于植物激素的类型和剂量,脂肪酸谱发生了显着变化。因此,使用植物激素的策略可能被证明是一种有意义的生物燃料生产方法。和细胞分裂素增加酶(过氧化氢酶和抗坏血酸过氧化物酶),清除活性氧化物质,从而调节微藻中的 ROS 稳态。由于植物激素的类型和剂量,脂肪酸谱发生了显着变化。因此,使用植物激素的策略可能被证明是一种有意义的生物燃料生产方法。和细胞分裂素增加酶(过氧化氢酶和抗坏血酸过氧化物酶),清除活性氧化物质,从而调节微藻中的 ROS 稳态。由于植物激素的类型和剂量,脂肪酸谱发生了显着变化。因此,使用植物激素的策略可能被证明是一种有意义的生物燃料生产方法。
更新日期:2020-08-01
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