Well-Defined DNA-Polymer Miktoarm Stars for Enzyme-Resistant Nanoflares and Carrier-Free Gene Regulation.,Bioconjugate Chemistry

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Well-Defined DNA-Polymer Miktoarm Stars for Enzyme-Resistant Nanoflares and Carrier-Free Gene Regulation.
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2020-02-12 , DOI: 10.1021/acs.bioconjchem.0c00017
Hui Li ₁ , Yang Li ₁ , Yue Xiao _{1,

2} , Bohan Zhang ₁ , Zehong Cheng ₁ , Jianqiao Shi ₁ , Jing Xiong ₁ , Zhaohui Li ₂ , Ke Zhang _{2,

3}

Affiliation

Herein, we report a star-architectured poly(ethylene glycol) (PEG)-oligonucleotide nanoconjugate of a well-defined molecular structure. Based upon fullerene C60 cores, each star bears precisely 1 DNA strand and 11 polymer chains. The elevated PEG density provides the DNA with steric selectivity: the DNA is significantly more resistant to nuclease digestion while remaining able to hybridize with a complementary sequence. The degree of resistance increases as the centers of mass for the DNA and fullerene are closer together. Such steric selectivity reduces protein-related background signals of the nanoflares synthesized from these miktoarm star polymers. Importantly, the stars improve cellular uptake and regulate gene expression as a non-cytotoxic, single-entity antisense agent without the need for a transfection carrier.

中文翻译：

定义明确的DNA聚合物Miktoarm星用于耐酶纳米杂化物和无载体基因调控。

在这里，我们报告一个明确定义的分子结构的星形结构的聚（乙二醇）（PEG）-寡核苷酸纳米共轭物。基于富勒烯C60核，每颗恒星恰好带有1条DNA链和11条聚合物链。升高的PEG密度为DNA提供了空间选择性：该DNA对核酸酶消化的抵抗力明显增强，同时仍然能够与互补序列杂交。随着DNA和富勒烯的质心越靠近，抗性程度越高。这种空间选择性降低了由这些miktoarm星形聚合物合成的纳米弹的蛋白质相关背景信号。重要的是，恒星无需转染载体即可作为一种非细胞毒性的单实体反义剂提高细胞摄取并调节基因表达。

更新日期：2020-02-13

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