Pumpkin seed oil (PSO) with carnauba wax and beeswax was used to develop nanostructured lipid carriers (NLC) loaded with a UV filter, Uvinul® A Plus B. The aims of the study were to optimize the concentration of PSO to develop a stable NLC formulation, determine storage stability of the NLC with and without PSO, and the synergistic effect of PSO‐NLC with UV filter for photoprotective properties. The physical properties of NLC were optimized based on the mean particle size, polydispersity index, and storage stability. The optimized NLC consisted 10% lipid phase (3.5% carnauba wax, 3.5% beeswax, and 3.0% PSO) and 90% aqueous phase. After optimization, Uvinul® A Plus B was added in the optimized PSO‐NLC to produce a photoprotective formulation. Uvinul® A Plus B consisted of both UVA (Diethylamino Hydroxybenzoyl Hexyl Benzoate) and UVB (Ethylhexyl Methoxycinnamate) filters. The NLC produced with PSO and Uvinul® A Plus B had mean particle size of 135 ± 2 nm and showed good physical stability under storage time. Besides that, the NLC produced also proven to have positive effect in enhancing the entrapment efficiency and drug loading, which were 82.86 ± 0.15% and 55.41 ± 0.04%, respectively, and showed sun protection factor value of 16.61 ± 3.45. The results indicated the presence of synergistic effect among the PSO‐NLC with Uvinul® A Plus B.

中文翻译：

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由巴西棕榈蜡，蜂蜡，南瓜籽油和紫外线过滤剂制成的具有光保护性能的新型纳米结构脂质载体的配制与表征

具有巴西棕榈蜡和蜂蜡的南瓜籽油（PSO）用于开发装有紫外线过滤器Uvinul®A Plus B的纳米结构脂质载体（NLC）。研究的目的是优化PSO的浓度以开发稳定的NLC配方，确定使用和不使用PSO的NLC的储存稳定性，以及使用UV滤光片的PSO-NLC对光保护性能的协同作用。基于平均粒径，多分散指数和储存稳定性优化了NLC的物理性质。优化的NLC包含10％脂质相（3.5％巴西棕榈蜡，3.5％蜂蜡和3.0％PSO）和90％水相。优化后，将Uvinul®A Plus B添加到优化的PSO-NLC中以产生光保护配方。U Plusul A Plus B由UVA（二乙氨基羟基苯甲酰苯甲酸己酯）和UVB（乙氧基甲氧基肉桂酸乙酯）过滤器组成。用PSO和Uvinul®A Plus B生产的NLC的平均粒径为135±2 nm，并且在储存时间下显示出良好的物理稳定性。除此之外，生产的NLC还被证明对提高包封效率和载药量有积极作用，分别为82.86±0.15％和55.41±0.04％，并且防晒系数值为16.61±3.45。结果表明PSO‐NLC与Uvinul®A Plus B之间存在协同效应。所产生的NLC也被证明对提高包封效率和载药量具有积极作用，分别为82.86±0.15％和55.41±0.04％，并且防晒系数值为16.61±3.45。结果表明PSO‐NLC与Uvinul®A Plus B之间存在协同效应。所产生的NLC也被证明对提高包封效率和载药量具有积极作用，分别为82.86±0.15％和55.41±0.04％，并且防晒系数值为16.61±3.45。结果表明PSO‐NLC与Uvinul®A Plus B之间存在协同效应。