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Molecular characterization and functional analysis of the vitellogenin receptor from eri silkworm, Samia ricini.
Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology ( IF 1.9 ) Pub Date : 2020-02-11 , DOI: 10.1016/j.cbpb.2020.110417 Dyna Susan Thomas 1 , Meenakshi Varma 1 , Chitra Manoharan 1 , Guru Prasad Rao 1 , Kunjupillai Vijayan 1 , Rakesh Kumar Mishra 1 , Ravikumar Gopalapillai 2 , Deepak Kumar Sinha 1
Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology ( IF 1.9 ) Pub Date : 2020-02-11 , DOI: 10.1016/j.cbpb.2020.110417 Dyna Susan Thomas 1 , Meenakshi Varma 1 , Chitra Manoharan 1 , Guru Prasad Rao 1 , Kunjupillai Vijayan 1 , Rakesh Kumar Mishra 1 , Ravikumar Gopalapillai 2 , Deepak Kumar Sinha 1
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The vitellogenin receptor (VgR) plays a critical role in egg development by mediating endocytosis of the major yolk protein precursor vitellogenin (Vg). Therefore, identifying the VgR of beneficial insects and its characterization could lead to the development of novel egg production strategies to enhance their commercial values. Here, we present the cloning, expression, and functional characterization of the VgR from an economically important eri silkworm, Samia ricini. The complete mRNA sequence was 6002 bp with an ORF of 5484 bp, encoding a protein of 1827 amino acids. Sequence analyses revealed that the SrVgR contained all of the conservative structural motifs characteristics of LDLR family members. The SrVgR was specifically expressed in the ovary, and the mRNA level increased steadily in pupal stages, reached its peak on day 9, and then declined to a bare minimum in adults. RNA interference (RNAi) clearly reduced the VgR transcript levels, disrupted the ovarian development resulting in malformed ovarioles and abnormal development of eggs. Taken together, these data provide conclusive evidence for the essential roles of VgR in insect reproduction.
中文翻译：
蚕蚕卵黄蛋白原受体的分子表征和功能分析。
卵黄蛋白原受体（VgR）通过介导主要蛋黄蛋白前体卵黄蛋白原（Vg）的胞吞作用在卵的发育中起关键作用。因此，鉴定有益昆虫的VgR及其特征可导致开发新颖的产蛋策略以提高其商业价值。在这里，我们介绍了来自经济上重要的蚕蚕（Samia ricini）的VgR的克隆，表达和功能表征。完整的mRNA序列为6002 bp，ORF为5484 bp，编码1827个氨基酸的蛋白质。序列分析表明，SrVgR包含LDLR家族成员的所有保守结构基序特征。SrVgR在卵巢中特异性表达，mRNA水平在p期稳定增加，并在第9天达到峰值。然后下降到成年人的最低限度。RNA干扰（RNAi）明显降低了VgR转录水平，破坏了卵巢发育，导致卵巢畸形和卵异常发育。综上所述，这些数据为VgR在昆虫繁殖中的重要作用提供了确凿的证据。
更新日期：2020-02-11
中文翻译：
蚕蚕卵黄蛋白原受体的分子表征和功能分析。
卵黄蛋白原受体（VgR）通过介导主要蛋黄蛋白前体卵黄蛋白原（Vg）的胞吞作用在卵的发育中起关键作用。因此，鉴定有益昆虫的VgR及其特征可导致开发新颖的产蛋策略以提高其商业价值。在这里，我们介绍了来自经济上重要的蚕蚕（Samia ricini）的VgR的克隆，表达和功能表征。完整的mRNA序列为6002 bp，ORF为5484 bp，编码1827个氨基酸的蛋白质。序列分析表明，SrVgR包含LDLR家族成员的所有保守结构基序特征。SrVgR在卵巢中特异性表达，mRNA水平在p期稳定增加，并在第9天达到峰值。然后下降到成年人的最低限度。RNA干扰（RNAi）明显降低了VgR转录水平，破坏了卵巢发育，导致卵巢畸形和卵异常发育。综上所述，这些数据为VgR在昆虫繁殖中的重要作用提供了确凿的证据。
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