ABSTRACT Current vaccines used for polio are live attenuated oral polio vaccine and inactivated polio vaccine. Recently, in India, the relapse of virulence was observed in attenuated viruses resulting in catastrophic effects. Therefore, the need for the development of multi-epitope subunit vaccine was realised and an immunoinformatics approach to design a multi-epitope subunit vaccine was conceived. Capsid proteins of all the three types of polio strains were utilised to predict major histocompatibility complex class-1 as well as class-2 epitopes. The subunit vaccine was designed with β-defensin at N-terminal followed by cytotoxic T-lymphocytes epitopes and helper T-lymphocytes epitopes connected by compatible linkers. The vaccine construct was further modelled and docked against TLR4 receptor. The high affinity of the construct towards the receptor was observed in the docking study and also substantiated by a 20 ns simulation of the complex. The vaccine construct was cloned in-silico for expression of the protein effectively in a prokaryotic system (Escherichia coli strain K12). Immuno-simulation of the construct was found to elicit immunoglobulin production effectively in the human body. This designed multi-epitope subunit vaccine is capable of immune response and further studies will help us understand the feasibility of this multi-epitope subunit vaccine.

中文翻译：

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利用脊髓灰质炎病毒衣壳蛋白通过免疫信息学方法设计基于多表位的亚单位疫苗

摘要 目前用于脊髓灰质炎的疫苗有口服脊髓灰质炎减毒活疫苗和脊髓灰质炎灭活疫苗。最近，在印度，在减毒病毒中观察到毒力复发，导致灾难性影响。因此，意识到开发多表位亚单位疫苗的需要，并构思了一种设计多表位亚单位疫苗的免疫信息学方法。所有三种脊髓灰质炎菌株的衣壳蛋白都被用来预测主要组织相容性复合体 1 类和 2 类表位。亚单位疫苗的设计是在 N 端使用 β-防御素，然后是细胞毒性 T 淋巴细胞表位和通过相容接头连接的辅助 T 淋巴细胞表位。疫苗构建体被进一步建模并对接TLR4受体。在对接研究中观察到构建体对受体的高亲和力，并通过复合物的 20 ns 模拟得到证实。为了在原核系统（大肠杆菌菌株K12）中有效地表达蛋白质，在计算机中克隆了疫苗构建体。发现构建体的免疫模拟可在人体内有效地引发免疫球蛋白的产生。这种设计的多表位亚单位疫苗能够产生免疫反应，进一步的研究将有助于我们了解这种多表位亚单位疫苗的可行性。发现构建体的免疫模拟可在人体内有效地引发免疫球蛋白的产生。这种设计的多表位亚单位疫苗能够产生免疫反应，进一步的研究将有助于我们了解这种多表位亚单位疫苗的可行性。发现构建体的免疫模拟可在人体内有效地引发免疫球蛋白的产生。这种设计的多表位亚单位疫苗能够产生免疫反应，进一步的研究将有助于我们了解这种多表位亚单位疫苗的可行性。