Transcriptomic analysis of cone snail venom duct tissue has permitted the identification of diverse conopressin/conophysin precursor sequences from seven distinct conus species. Multiple precursor isoforms are present in C.monile, C.lividus and C.loroisii. Aqueous extracts of the venom duct tissue from C.monile yield a band, at ~ 15-20 kDa on SDS-PAGE. In-gel trypsin digestion, followed by mass spectrometry establishes the presence of two distinct conopressin/conophysin isoforms that differ at position 8 in the predicted conopressin nonapeptide sequence. Mass spectrometric analysis of aqueous extracts revealed the presence of four conopressin related peptides, whose sequences could be deduced from MS/MS fragmentation patterns. The four sequences determined in this study are CFIRNCPKG*, CFIRNCPEG*, CFIRNCPK* and CFIRNCPE* (∗ indicates amide), which were further confirmed by comparison with chemically synthesized peptides. A conophysin with a mass of 9419.7 Da was also detected, corresponding to one of the isoforms revealed by the transcriptome data. Complete conservation of fourteen Cys residues and the key residues involved in peptide hormone binding is established by comparison of conophysin sequences, with the crystallographically characterized sequence of bovine neurophysin, in complex with vasopressin. A survey of available sequences for oxytocin/vasopressin peptides in both vertebrates and invertebrates establishes the conopressins as a distinct group in this family. C-terminal amidated, truncated conopressin analogs may arise by alternate post-translational processing.

锥蜗牛毒液导管组织的转录组学分析已允许从七个不同的圆锥体物种中鉴定出不同的conopressin / conophysin前体序列。C.monile，C.lividus和C.loroisii中存在多种前体同工型。梭菌毒液导管组织的水提物在SDS-PAGE上产生约15-20 kDa的条带。凝胶内胰蛋白酶消化，然后进行质谱分析，确定了两种不同的降压药/降压药同工型的存在，它们在预测的降压药非肽序列的8位不同。水提取物的质谱分析揭示了四种与降压素相关的肽的存在，其序列可从MS / MS片段谱图推导出。在这项研究中确定的四个序列是CFIRNCPKG *，CFIRNCPEG *，CFIRNCPK *和CFIRNCPE *（*表示酰胺），通过与化学合成的肽进行比较进一步得到了证实。还检测到质量为9419.7 Da的圆锥体，对应于转录组数据揭示的同工型之一。通过比较锥蛋白序列与牛神经素的晶体学表征序列与血管加压素的复合物，可以确定十四个半胱氨酸残基和参与肽激素结合的关键残基的完全保守性。对脊椎动物和无脊椎动物中催产素/加压素肽的可用序列进行的调查确定，加压素是该家族中的一个独特群体。C末端酰胺化，截短的conopressin类似物可能通过交替的翻译后加工产生。对脊椎动物和无脊椎动物中催产素/加压素肽的可用序列进行的调查确定，加压素是该家族中的一个独特群体。C末端酰胺化，截短的conopressin类似物可能通过交替的翻译后加工产生。对脊椎动物和无脊椎动物中催产素/加压素肽的可用序列进行的调查确定，加压素是该家族中的一个独特群体。C末端酰胺化，截短的conopressin类似物可能通过交替的翻译后加工产生。