Transfection, the process of introducing purified nucleic acids into cells, and viral transduction, viral-mediated nucleic acid transfer, are two commonly utilized techniques for gene delivery in the research setting. Transfection allows purified nucleic acid to be introduced into target cells through chemical-based techniques, nonchemical methods or particle-based methods, while viral transduction employs genomes or vectors based on adenoviruses, retroviruses (e.g. lentiviruses), adeno-associated viruses, or hybrid viruses. Transfected DNAs are often tested for potential effects on subsequent transduction, but it is not clear whether transfection itself rather than the particular nucleic acid being introduced might impact subsequent viral transfection. We observed a significant association between successfully transfected mobilized peripheral blood CD34+ human stem and progenitor cells (HSPCs) and permissiveness to subsequent lentiviral transduction, which was not evident in other cells such as 293 T cells and Jurkat cells. This association, apparently specific to CD34+ human stem and progenitor cells (HSPCs), is critical to both research and clinical applications as these cells are a frequent target of transfection and viral transduction owing to the durable nature of these cells in living systems. This finding may also present a significant opportunity to enhance the success of viral transduction for clinical applications.

中文翻译：

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成功转染的原代外周动员人CD34 +造血干细胞和祖细胞（HSPC）显示出对逆转录病毒感染的敏感性增加。

转染是将纯化的核酸引入细胞的过程，而病毒转导是指病毒介导的核酸转移，是研究环境中两种常用的基因传递技术。转染允许通过基于化学的技术，非化学方法或基于颗粒的方法将纯化的核酸引入靶细胞，而病毒转导采用基于腺病毒，逆转录病毒（例如慢病毒），腺相关病毒或杂交病毒的基因组或载体。通常测试转染的DNA对后续转导的潜在影响，但尚不清楚转染本身而不是所引入的特定核酸是否会影响后续的病毒转染。我们观察到成功转染的动员外周血CD34 +人干细胞和祖细胞（HSPC）与随后的慢病毒转导的允许性之间存在显着关联，这在其他细胞（如293 T细胞和Jurkat细胞）中并不明显。这种关联显然对CD34 +人干细胞和祖细胞（HSPC）具特异性，对研究和临床应用都是至关重要的，因为这些细胞由于其在生命系统中的持久性而成为转染和病毒转导的常见靶标。这一发现也可能为增强病毒转导在临床应用中的成功提供了重要的机会。在其他细胞（例如293 T细胞和Jurkat细胞）中并不明显。这种关联显然对CD34 +人干细胞和祖细胞（HSPC）具特异性，对研究和临床应用都是至关重要的，因为这些细胞由于其在生命系统中的持久性而成为转染和病毒转导的常见靶标。这一发现也可能为增强病毒转导在临床应用中的成功提供了重要的机会。在其他细胞（例如293 T细胞和Jurkat细胞）中并不明显。这种关联显然对CD34 +人干细胞和祖细胞（HSPC）具特异性，对研究和临床应用都是至关重要的，因为这些细胞由于其在生命系统中的持久性而成为转染和病毒转导的常见靶标。这一发现也可能为增强病毒转导在临床应用中的成功提供了重要的机会。