Leaf herbivory on tea plants (Camellia sinensis) by tea geometrids (Ectropis oblique) can cause severe yield loss and quality damage for tea. In previous work, we discovered that leaf herbivory triggered systemic carbon depletion in undamaged roots to enhance resource investment for local defense induced in damaged leaves. Here, we investigated the dynamics of amino acids in the local and systemic responses and the roles of nitrogen resource reallocation for the inducible defense in tea plants in response to leaf herbivory. The comparative analysis of the dynamics of flavonoids, caffeine, theanine and basic amino acids at metabolic and transcriptome levels revealed that leaf herbivory triggered the differential reconfiguration of these amino acid-derived defensive metabolites and nitrogenous primary metabolism between the local and systemic responses. The tight association of the metabolism and reallocation of amino acids with the activation of defensive secondary metabolism indicated that the systemic nitrogen reallocation played a potentially important role for the resource investment in tea plant resistance against leaf herbivory. This study provided an extended understanding of the role of systemic nitrogen reallocation for the interaction of tea plants and geometrids and the root-mediated resource-based resistance strategy employed by tea plants in response to leaf herbivory.

中文翻译：

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茶树（Camellia sinensis）对斜草ivo食草对叶片食草的诱导诱导防御作用的局部和全身氨基酸代谢变化。

茶几何体（斜c）对茶树（茶树）的叶片食草会导致严重的产量损失和茶叶质量受损。在先前的工作中，我们发现叶片食草会在未损坏的根部触发系统性碳耗竭，从而增加资源投资，以用于受损叶片诱导的局部防御。在这里，我们调查了氨基酸在局部和系统响应中的动态，以及氮素资源的重新分配在茶树对叶草的响应中对诱导型防御的作用。黄酮类，咖啡因，茶氨酸和碱性氨基酸在代谢和转录组水平上显示，叶片食草性引发了这些氨基酸衍生的防御性代谢产物和局部和全身反应之间的氮素初级代谢的差异重配置。氨基酸的代谢和重新分配与防御性次级代谢的激活紧密相关，这表明系统的氮重新分配对于茶树抗叶草性的资源投资起着潜在的重要作用。这项研究提供了对系统氮分配在茶树和几何体相互作用中的作用以及茶树对叶片食草性反应所采用的根系介导的基于资源的抗性策略的扩展理解。氨基酸的代谢和重新分配与防御性次级代谢的激活紧密相关，这表明系统的氮重新分配对于茶树抗叶草性的资源投资起着潜在的重要作用。这项研究提供了对系统氮分配在茶树和几何体相互作用中的作用以及茶树对叶片食草性反应所采用的根系介导的基于资源的抗性策略的扩展理解。氨基酸的代谢和重新分配与防御性次级代谢的激活紧密相关，这表明系统的氮重新分配对于茶树抗叶草性的资源投资起着潜在的重要作用。这项研究提供了对系统氮分配在茶树和几何体相互作用中的作用以及茶树对叶片食草性反应所采用的根系介导的基于资源的抗性策略的扩展理解。