BACKGROUND AIMS Autologous hematopoietic stem cell transplantation (AHSCT) is an alternative for multiple sclerosis (MS) patients who do not respond to conventional treatment. Mobilization kinetics of CD34+ cells in MS patients has not been studied. METHODS Patients with MS mobilized with granulocyte colony-stimulating factor (G-CSF) and cyclophosphamide (Cy) were prospectively studied. Three counts of CD34+ cells were done in peripheral blood: at baseline before mobilization, at the start, and immediately at the end of apheresis. Complete blood counts were performed at the times of CD34+ cell counting. Standard statistical descriptive analysis of MS patients' salient features was performed, and after log 10 transformation of the data, Pearson test was performed to assess correlation between variables and CD34+ cell count. In addition, multiple linear regression of relevant data was carried out for multivariate analysis. RESULTS Data of 51 consecutive MS patients with median age of 48 (31-64) years were analyzed. The CD34+ cell count increased 26-fold after mobilization. During large volume leukapheresis (LVL), the number of CD34+ cells in peripheral blood increased from 51.29 CD34+/μL at the start to 62.3 CD34+/μL at the end. A negative correlation between CD34+ cell count after leukapheresis and age (r = -0.32, P = 0.02) was observed. Neither the CD34+ baseline count nor sex correlated with the CD34+ count in peripheral blood immediately at the end of apheresis. CONCLUSIONS Mobilization with G-CSF and Cy in MS patients resulted in effective CD34+ hematoprogenitors release from the bone marrow and in intra-apheresis recruitment.

中文翻译：

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G-CSF 和环磷酰胺刺激 CD34+ 造血干细胞在接受自体移植的多发性硬化患者中的动员动力学

背景 目的 自体造血干细胞移植 (AHSCT) 是对常规治疗无反应的多发性硬化 (MS) 患者的替代方法。尚未研究 MS 患者中 CD34+ 细胞的动员动力学。方法 前瞻性研究了用粒细胞集落刺激因子 (G-CSF) 和环磷酰胺 (Cy) 动员的 MS 患者。在外周血中进行了三项 CD34+ 细胞计数：在动员前的基线、开始时和单采结束时。在 CD34+ 细胞计数时进行全血计数。对 MS 患者的显着特征进行标准统计描述性分析，并对数据进行 log 10 转换后，进行 Pearson 检验以评估变量与 CD34+ 细胞计数之间的相关性。此外，对相关数据进行多元线性回归进行多元分析。结果 分析了 51 名中位年龄为 48 (31-64) 岁的连续 MS 患者的数据。动员后 CD34+ 细胞计数增加了 26 倍。大容量白细胞去除术（LVL）期间，外周血CD34+细胞数从开始时的51.29 CD34+/μL增加到结束时的62.3 CD34+/μL。观察到白细胞去除术后 CD34+ 细胞计数与年龄呈负相关（r = -0.32，P = 0.02）。CD34+ 基线计数和性别均与单采结束时外周血中的 CD34+ 计数不相关。结论 MS 患者中 G-CSF 和 Cy 的动员导致 CD34+ 造血祖细胞从骨髓中有效释放和血液分离内募集。