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Gold Nanobones Enhanced Ultrasensitive Surface-Enhanced Raman Scattering Aptasensor for Detecting Escherichia coli O157:H7.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-02-10 , DOI: 10.1021/acssensors.9b02600
Shuaishuai Zhou 1 , Chang Lu 1 , Yuzhi Li 1 , Li Xue 2 , Chengying Zhao 1 , Guifang Tian 1 , Yuming Bao 1 , Longhua Tang 3 , Jianhan Lin 2 , Jinkai Zheng 1
Affiliation  

Sensitive, robust, and highly specific detection of Escherichia coli O157:H7, one of the most hazardous foodborne pathogens and the cause of numerous diseases, is needed to ensure public health. Herein, a one-pot step method is reported for the preparation of multifunctional gold nanobones (NBs) (GNRApt-1+RhB) from gold nanorods (GNRs) comediated by an aptamer (Apt-1) and the signal molecule rhodamine B (RhB) for surface-enhanced Raman scattering detection of E. coli O157:H7. The characterized result showed that Apt-1 and RhB were embedded in the gold NBs, and then, this combination exhibited good recognition, excellent stability, and significant Raman signal intensity enhancement. The Raman enhancement derived from a strong electromagnetic field distribution with the locations at the apex of both ends of the GNRApt-1+RhB and the signal stability was because of the firm embedment of Apt-1 (poly A20 + E. coli O157:H7 aptamers) and RhB on the surface of the GNRApt-1+RhB. Optimization experiments established that surface-enhanced Raman-scattered RhB absorption at 1350 cm-1 had a strong linear relationship (y = 180.30x - 61.49; R2 = 0.9982) with E. coli O157:H7 concentration over the range of 10-10,000 cfu/mL with a limit of detection of 3 cfu/mL. This novel aptasensor sensitively detects E. coli O157:H7 and has great promise for food pathogenic bacteria detection.

中文翻译:

金纳米颗粒增强型超灵敏表面增强拉曼散射自适应传感器,用于检测大肠杆菌O157:H7。

为了确保公众健康,需要对大肠杆菌O157:H7(最危险的食源性病原体和多种疾病的起因)进行灵敏,强大且高度特异性的检测。本文报道了一种一锅法,由适体(Apt-1)和信号分子若丹明B(RhB)介导的金纳米棒(GNR)制备多功能金纳米骨(NBs)(GNRApt-1 + RhB) )用于大肠杆菌O157:H7的表面增强拉曼散射检测。表征结果表明,Apt-1和RhB嵌入金NB中,然后这种组合显示出良好的识别性,出色的稳定性和显着的拉曼信号强度增强。拉曼增强来自强大的电磁场分布,其分布在GNRApt-1 + RhB两端的顶点,并且信号稳定性是由于Apt-1的牢固嵌入(poly A20 +大肠杆菌O157:H7适体)和GNRApt-1 + RhB表面上的RhB。优化实验表明,表面增强的拉曼散射RhB在1350 cm-1处的吸收与大肠杆菌O157:H7的浓度在10-10,000 cfu范围内具有很强的线性关系(y = 180.30x-61.49; R2 = 0.9982)。 / mL,检出限为3 cfu / mL。这种新颖的适体传感器可灵敏地检测大肠杆菌O157:H7,并有望用于食品致病菌的检测。优化实验表明,表面增强的拉曼散射RhB在1350 cm-1处的吸收与大肠杆菌O157:H7的浓度在10-10,000 cfu范围内具有很强的线性关系(y = 180.30x-61.49; R2 = 0.9982)。 / mL,检出限为3 cfu / mL。这种新颖的适体传感器可灵敏地检测大肠杆菌O157:H7,并有望用于食品致病菌的检测。优化实验表明,表面增强的拉曼散射RhB在1350 cm-1处的吸收与大肠杆菌O157:H7的浓度在10-10,000 cfu范围内具有很强的线性关系(y = 180.30x-61.49; R2 = 0.9982)。 / mL,检出限为3 cfu / mL。这种新颖的适体传感器可灵敏地检测大肠杆菌O157:H7,并有望用于食品致病菌的检测。
更新日期:2020-02-19
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