Simultaneous multiplane imaging with reverberation two-photon microscopy,Nature Methods

当前位置： X-MOL 学术 › Nat. Methods › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Simultaneous multiplane imaging with reverberation two-photon microscopy
Nature Methods ( IF 36.1 ) Pub Date : 2020-02-10 , DOI: 10.1038/s41592-019-0728-9
Devin R Beaulieu ₁ , Ian G Davison _{2,

3} , Kıvılcım Kılıç ₄ , Thomas G Bifano ₃ , Jerome Mertz _{3,

4}

Affiliation

Multiphoton microscopy has gained enormous popularity because of its unique capacity to provide high-resolution images from deep within scattering tissue. Here, we demonstrate video-rate multiplane imaging with two-photon microscopy by performing near-instantaneous axial scanning while maintaining three-dimensional micrometer-scale resolution. Our technique, termed reverberation microscopy, enables the monitoring of neuronal populations over large depth ranges and can be implemented as a simple add-on to a conventional design.

中文翻译：