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Simultaneous multiplane imaging with reverberation two-photon microscopy
Nature Methods ( IF 36.1 ) Pub Date : 2020-02-10 , DOI: 10.1038/s41592-019-0728-9
Devin R Beaulieu 1 , Ian G Davison 2, 3 , Kıvılcım Kılıç 4 , Thomas G Bifano 3 , Jerome Mertz 3, 4
Affiliation  

Multiphoton microscopy has gained enormous popularity because of its unique capacity to provide high-resolution images from deep within scattering tissue. Here, we demonstrate video-rate multiplane imaging with two-photon microscopy by performing near-instantaneous axial scanning while maintaining three-dimensional micrometer-scale resolution. Our technique, termed reverberation microscopy, enables the monitoring of neuronal populations over large depth ranges and can be implemented as a simple add-on to a conventional design.



中文翻译:

用混响双光子显微镜同时进行多平面成像

多光子显微镜因其从散射组织深处提供高分辨率图像的独特能力而获得了极大的普及。在这里,我们通过执行近瞬时轴向扫描,同时保持三维微米级分辨率,展示了双光子显微镜的视频速率多平面成像。我们的技术,称为混响显微镜,能够监测大深度范围内的神经元群体,并且可以作为传统设计的简单附加实现。

更新日期:2020-02-10
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