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Development and characterization of functionalized glyco thiolate capped gold nanoparticles for biological applications
RSC Medicinal Chemistry ( IF 4.1 ) Pub Date : 2020/01/28 , DOI: 10.1039/c9md00493a Christian K Adokoh 1 , Frankline K Keter 2 , Henok H Kinfe 1 , Robert Tshikhudo 2 , James Darkwa 1
RSC Medicinal Chemistry ( IF 4.1 ) Pub Date : 2020/01/28 , DOI: 10.1039/c9md00493a Christian K Adokoh 1 , Frankline K Keter 2 , Henok H Kinfe 1 , Robert Tshikhudo 2 , James Darkwa 1
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Glyco-gold nanoparticles (AuNPs) in aqueous dispersions were prepared by two approaches, namely direct reduction and ligand substitution methods. In the direct method, potassium salts of glyco thiols, with the general formula (C6H11O6)NH(CH2)nCH2SK (where L1, n = 1; L2, n = 2; L3, n = 3, L4, n = 4; L5, n = 5), were used as reducing and capping agents to give the glyco thiolate capped gold nanoparticles (AuNPs G1–G5); meanwhile in the ligand exchange experiments, L1–L5 and their acetylated forms (L6–L8) replaced citrate ions in citrate-capped gold nanoparticles to give additional AuNPs G6–G11. UV-visible spectroscopy, surface charge (ζ-potential,) measurements and transmission electron microscopy (TEM) were used for physical and chemical characterization of all the resultant AuNPs. The ζ-potential studies of AuNPs prepared through the direct method revealed that the surface charge is dependent on the length of the alkyl unit of (C6H11O6)NH(CH2)nCH2S− ligands. TEM images of the acetylated and non-acetylated glyco thiolate capped gold nanoparticles (AuNPs G6–G11) prepared via the ligand exchange method indicate that the size and shape of the gold nanoparticles remained the same as those of the citrate-capped gold nanoparticles used to prepare them. Selected AuNPs were tested on peripheral blood mononuclear cells (PBMCs) and the A549 cancer cell line to investigate their respective toxicity and cytotoxicity profiles. All AuNPs showed indiscriminate activity against both PBMCs and A4549 cells, although the gold nanoparticles having an acetylated glyco moiety with an amino propyl thiol linker as the ligand (G10) prepared via the citrate exchange method had better selectivity (PBMCs >59 mg mL−1 and for A549 ∼7 μg mL−1).
中文翻译：
用于生物应用的功能化乙二醇硫醇封端金纳米颗粒的开发和表征
水分散体中的糖基金纳米粒子（AuNPs）通过两种方法制备，即直接还原法和配体取代法。在直接法中，糖硫醇的钾盐，通式为(C 6 H 11 O 6 )NH(CH 2 ) n CH 2 SK(其中L1，n =1；L2，n =2；L3，n = 3、L4，n = 4；L5，n = 5)，用作还原剂和封端剂，得到乙二醇硫醇封端的金纳米颗粒（AuNPs G1 – G5）；同时在配体交换实验中，L1 – L5及其乙酰化形式（L6 – L8）取代了柠檬酸盐封端的金纳米颗粒中的柠檬酸根离子，得到额外的 AuNPs G6 – G11。使用紫外-可见光谱、表面电荷（电位）测量和透射电子显微镜 (TEM) 对所有所得 AuNP 进行物理和化学表征。通过直接法制备的 AuNPs 的z电位研究表明，表面电荷取决于 (C 6 H 11 O 6 )NH(CH 2 ) n CH 2 S -配体的烷基单元的长度。通过配体交换法制备的乙酰化和非乙酰化乙醇硫醇封端金纳米颗粒（AuNPs G6 – G11）的TEM图像表明，金纳米颗粒的尺寸和形状与用于制备柠檬酸盐封端的金纳米颗粒相同。让他们做好准备。选定的 AuNP 在外周血单核细胞 (PBMC) 和 A549 癌细胞系上进行了测试，以研究它们各自的毒性和细胞毒性特征。所有AuNPs都对PBMCs和A4549细胞表现出不加区别的活性，尽管通过柠檬酸盐交换法制备的具有乙酰化糖部分和氨基丙硫醇连接体作为配体（ G10）的金纳米颗粒具有更好的选择性（PBMCs>59 mg mL -1对于 A549 ∼7 μg mL -1 )。
更新日期：2020-02-27
中文翻译：
用于生物应用的功能化乙二醇硫醇封端金纳米颗粒的开发和表征
水分散体中的糖基金纳米粒子（AuNPs）通过两种方法制备，即直接还原法和配体取代法。在直接法中，糖硫醇的钾盐，通式为(C 6 H 11 O 6 )NH(CH 2 ) n CH 2 SK(其中L1，n =1；L2，n =2；L3，n = 3、L4，n = 4；L5，n = 5)，用作还原剂和封端剂，得到乙二醇硫醇封端的金纳米颗粒（AuNPs G1 – G5）；同时在配体交换实验中，L1 – L5及其乙酰化形式（L6 – L8）取代了柠檬酸盐封端的金纳米颗粒中的柠檬酸根离子，得到额外的 AuNPs G6 – G11。使用紫外-可见光谱、表面电荷（电位）测量和透射电子显微镜 (TEM) 对所有所得 AuNP 进行物理和化学表征。通过直接法制备的 AuNPs 的z电位研究表明，表面电荷取决于 (C 6 H 11 O 6 )NH(CH 2 ) n CH 2 S -配体的烷基单元的长度。通过配体交换法制备的乙酰化和非乙酰化乙醇硫醇封端金纳米颗粒（AuNPs G6 – G11）的TEM图像表明，金纳米颗粒的尺寸和形状与用于制备柠檬酸盐封端的金纳米颗粒相同。让他们做好准备。选定的 AuNP 在外周血单核细胞 (PBMC) 和 A549 癌细胞系上进行了测试，以研究它们各自的毒性和细胞毒性特征。所有AuNPs都对PBMCs和A4549细胞表现出不加区别的活性，尽管通过柠檬酸盐交换法制备的具有乙酰化糖部分和氨基丙硫醇连接体作为配体（ G10）的金纳米颗粒具有更好的选择性（PBMCs>59 mg mL -1对于 A549 ∼7 μg mL -1 )。
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